CNF1/CNF2 Antibody (JC4) - Azide Free

Novus Biologicals | Catalog # NBP3-11249

Novus Biologicals
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Key Product Details

Species Reactivity

E. coli

Applications

Western Blot, Immunoassay, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # JC4

Format

Azide Free
View all CNF1/CNF2 Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli.

Epitope

Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<24 EU/mg

Applications for CNF1/CNF2 Antibody (JC4) - Azide Free

Application
Recommended Usage

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 or CNF2 to be inactivated.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CNF1/CNF2

The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. CNF1 and 2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal binding domain, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain. JC4 neutralizes only CNF1.

Alternate Names

Cytotoxic necrotizing factor, Cytotoxic necrotizing factor type 1, Cytotoxic necrotizing factor type 2

Additional CNF1/CNF2 Products

Product Documents for CNF1/CNF2 Antibody (JC4) - Azide Free

Certificate of Analysis

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Product Specific Notices for CNF1/CNF2 Antibody (JC4) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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