CNOT3 Antibody (4B8) - Azide and BSA Free

Novus Biologicals | Catalog # H00004849-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B8

Format

Azide and BSA Free
View all CNOT3 Primary Antibodies »

Product Specifications

Immunogen

CNOT3 (NP_055331, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKT

Localization

Cytoplasmic and Nuclear

Specificity

CNOT3 - CCR4-NOT transcription complex, subunit 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CNOT3 Antibody (4B8) - Azide and BSA Free

Western Blot: CNOT3 Antibody (4B8) [H00004849-M01]

Western Blot: CNOT3 Antibody (4B8) [H00004849-M01]

Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody (M01), clone 4B8.Lane 1: CNOT3 transfected lysate(82 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01]

Immunocytochemistry/ Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01]

Immunocytochemistry/Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of monoclonal antibody to CNOT3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: CNOT3 Antibody (4B8) [H00004849-M01]

Western Blot: CNOT3 Antibody (4B8) [H00004849-M01]

Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - CNOT3 monoclonal antibody (M01), clone 4B8 Analysis of CNOT3 expression in Hela S3 NE.
ELISA: CNOT3 Antibody (4B8) [H00004849-M01]

ELISA: CNOT3 Antibody (4B8) [H00004849-M01]

ELISA: CNOT3 Antibody (4B8) [H00004849-M01] - Detection limit for recombinant GST tagged CNOT3 is 0.3 ng/ml as a capture antibody.

Applications for CNOT3 Antibody (4B8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27746116).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CNOT3

CNOT3 is a protein component of CCR4 NOT protein complex, a general transcription regulation complex. It has 3 named isoforms produced by alternative splicing. Yeast CCR4 NOT is a global regulator of RNA polymerase II transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional proteins like yeast CAF1.

Alternate Names

CCR4-NOT transcription complex, subunit 3, KIAA0691CCR4-associated factor 3, LENG2yeast) homolog, Leukocyte receptor cluster member 2

Entrez Gene IDs

4849 (Human)

Gene Symbol

CNOT3

OMIM

604910 (Human)

UniProt

NP_055331 (Human)

Additional CNOT3 Products

Product Documents for CNOT3 Antibody (4B8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CNOT3 Antibody (4B8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CNOT3 Antibody (4B8) - Azide and BSA Free

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Protocols

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FAQs

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