CNOT3 Antibody

Novus Biologicals | Catalog # NBP1-21396

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CNOT3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNOT3 Antibody

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-21396 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate
Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - Human testicular seminoma. Antibody used at a dilution of 1:200 (1ug/ml).
Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells.
Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells.
Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - of human ovarian carcinoma. Dilution of 1:1,000 (1ug/ml).
Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CNOT3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for CNOT3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CNOT3

CNOT3 (CCR4-NOT transcription complex subunit 3) is a component of the CCR4-NOT core complex that functions as a general transcriptional regulator. In a genome-wide siRNA screen for genes essential for mouse embryonic stem cell self-renewal, CNOT3 was identified and found to co-occupy gene promoters with c-Myc and Zfx.

Alternate Names

CCR4-NOT transcription complex, subunit 3, KIAA0691CCR4-associated factor 3, LENG2yeast) homolog, Leukocyte receptor cluster member 2

Entrez Gene IDs

4849 (Human)

Gene Symbol

CNOT3

UniProt

O75175 (Human)

Additional CNOT3 Products

Product Documents for CNOT3 Antibody

Certificate of Analysis

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Product Specific Notices for CNOT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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