Q: Do you have neutralizing antibodies for anti-mouse Thrombin?

A: We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1407

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Cell Lysate, Plasma, Serum (50 uL)

Sensitivity

0.24 ng/mL

Assay Range

0.391 to 100 ng/mL

View all Coagulation Factor II/Thrombin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Porcine

Description

F2 (Pig) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of pig F2.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.8%

Inter-Assay Precision (Precision between assays) CV% 7.4%

Recovery for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Recovery

96%

Scientific Data Images for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

ELISA: Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [KA1407]

ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

Kit Contents for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Biotinylated Swine Prothrombin Antibody (100x): A 100-fold concentrated biotinylated polyclonal antibody against swine prothrombin.
Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
Swine Prothrombin Microplate: A 96-well polystyrene microplate coated with a monoclonal antibody against swine prothrombin.
Swine Prothrombin Standard: Swine prothrombin in a buffered protein base, lyophilized.
Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor II/Thrombin

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Alternate Names

F2, Thrombin

Gene Symbol

Additional Coagulation Factor II/Thrombin Products

Product Documents for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Download Product Datasheets

Download F2 (Pig) ELISA Kit

Product Specific Notices for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Coagulation

Serine Proteases and Regulators

Citations for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

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