Q: Do you have neutralizing antibodies for anti-mouse Thrombin?

A: We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82491

Submit a review

Product Documents

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Coagulation Factor II/Thrombin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat Coagulation Factor II/Thrombin in samples. No Significant cross-reactivity or interference between Rat Coagulation Factor II/Thrombin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.15

Inter-Assay Precision (Precision between assays) CV% 4.46

Recovery for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Recovery

85-106%

Linearity

Scientific Data Images for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] -

ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] - Standard Curve Reference

Kit Contents for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor II/Thrombin

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Alternate Names

F2, Thrombin

Gene Symbol

Additional Coagulation Factor II/Thrombin Products

Product Documents for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Download SDS

Product Specific Notices for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Coagulation

Serine Proteases and Regulators

Customer Reviews for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) and earn rewards!

Have you used Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image