Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82464

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat Coagulation Factor III/Tissue Factor in samples. No Significant cross-reactivity or interference between Rat Coagulation Factor III/Tissue Factor and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 9.19

Inter-Assay Precision (Precision between assays) CV% 10.27

Recovery for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Recovery

85-101%

Linearity

Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Scientific Data Images for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

ELISA: Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric) [NBP2-82464] -

ELISA: Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric) [NBP2-82464] - Standard Curve Reference

Kit Contents for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor III/Tissue Factor

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

F3

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Rat Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

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  • Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

    A: Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail