Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

A: Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-42890

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Product Documents

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Plasma (100 uL)

Sensitivity

0.054 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

View all Coagulation Factor III/Tissue Factor ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Porcine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Recovery

79 - 94% (example only; lot dependent)

Kit Contents for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor III/Tissue Factor

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - NBP3-42890

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Coagulation

Endothelial Cell Markers

Monocyte Markers

Serine Proteases and Regulators

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