COASY Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29017

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COASY Primary Antibodies »

Product Specifications

Immunogen

Between 225 and 275

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COASY Antibody - BSA Free

COASY Antibody

Immunoprecipitation:COASY AntibodyNBP3-29017] -

Immunoprecipitation:COASY AntibodyNBP3-29017] -Detection of human COASY by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COASY antibody NBP3-29017 (lot NBP3-29017-1) used for IP at 6 µg per reaction. COASY was also immunoprecipitated by rabbit anti-COASY antibody. For blotting immunoprecipitated COASY, NBP3-29017 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for COASY Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, POV-2, PPAT

Gene Symbol

COASY

Additional COASY Products

Product Documents for COASY Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for COASY Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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