Common beta Chain Antibody (CSL311) - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28274

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 Clone # CSL311 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all Common beta Chain Primary Antibodies »

Product Specifications

Immunogen

CSF2Rb / CD131

Clonality

Monoclonal

Host

Human

Isotype

IgG4

Description

The heavy chain type is huIgG4, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Patent #: WO2023173173A

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

Common beta Chain Antibody (CSL311)

Functional: Common beta Chain Antibody (CSL311)[NBP3-28274] -

GM-CSF-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 4.105 nM.
Common beta Chain Antibody (CSL311)

ELISA: Common beta Chain Antibody (CSL311)[NBP3-28274] -

Immobilized human CSF2RB His at 2 ug/mL can bind Common beta Chain Antibody (CSL311), EC50=0.0122 ug/mL.
Common beta Chain Antibody (CSL311)

Functional: Common beta Chain Antibody (CSL311)[NBP3-28274] -

IL-3-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 0.2109 nM.
Common beta Chain Antibody (CSL311)

Flow Cytometry: Common beta Chain Antibody (CSL311)[NBP3-28274] -

Human CSF2RB HEK293 cells were stained with Common beta Chain Antibody (CSL311) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.049 ug/mL.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Common beta Chain

Interleukin-3 receptor (IL-3R) is a heterodimer cytokine composed of alpha chain and beta chain. IL-3R beta subunit (CD131, beta C) is a common shared beta chain of the receptor for granulocyte- macrophage colony-stimulating factor (GM-CSF) and IL-5 (1). While the beta chain can not bind to the ligand alone, but it is essential for high affinity ligand binding (2). The cytoplasmic portion of the beta chain contains the major domains necessary for ligand-induced proliferation (3). IL-3R beta is expressed in neutrophil, eosinophil, monocyte, and hematpoietic stem cells. A point mutation leading to defective IL-3R beta expression has been associated to Human pulmonary alveolar proteinosis (PAP), a rare cause of respiratory failure (4).

Alternate Names

CD131, CSF2RB, GM-CSF R beta, IL-3 R beta, IL-5 R beta

Gene Symbol

CSF2RB

UniProt

P32927

Additional Common beta Chain Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail