Q: I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?

A: We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

COMP/Thrombospondin-5 Antibody Pair [HRP]

Novus Biologicals | Catalog # NBP2-79472

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

39.06-2500 pg/ml (example only; lot dependent)

Sensitivity

39.1 pg/ml (example only; lot dependent)

Reactivity

Human

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Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human COMP/Thrombospondin-5.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for COMP/Thrombospondin-5 Antibody Pair [HRP]

Sandwich ELISA: COMP/Thrombospondin-5 Antibody Pair [Biotin] [NBP2-79472] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for COMP/Thrombospondin-5 Antibody Pair [HRP]

Mouse Monoclonal Capture Antibody
Mouse Monoclonal Detection Antibody (HRP-conjugated)
Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for COMP/Thrombospondin-5 Antibody Pair [HRP]

Download Product Datasheets

Download Manual - COMP/Thrombospondin-5 Antibody Pair [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for COMP/Thrombospondin-5 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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