Q: I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?

A: We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82141

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all COMP/Thrombospondin-5 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse COMP/Thrombospondin-5 in samples. No Significant cross-reactivity or interference between Mouse COMP/Thrombospondin-5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 6%

Inter-Assay Precision (Precision between assays) CV% 6%

Recovery for Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Recovery

average = 98% for serum; average = 96% for EDTA plasma; average = 100% for cell culture media

Linearity

Scientific Data Images for Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

ELISA: Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82141] -

ELISA: Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82141] - Standard Curve Reference

Kit Contents for Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

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Product Specific Notices for Mouse COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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