Complement C4d Antibody (C4D204)

Novus Biologicals | Catalog # NBP2-34234

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C4D204
View all Complement C4d Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Complement Complement C4d protein

Localization

Intracytoplasmic vacuoles of endothelial cells and Secreted

Marker

Acute Humoral Rejection Marker

Specificity

This monoclonal antibody is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34581)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Complement C4d Antibody (C4D204)

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (20X) stained with Complement 4d MAb (C4D204).
Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (10X) stained with Complement 4d MAb (C4D204).
Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (40X) stained with Complement 4d Ab (C4D204).
Complement C4d Antibody (C4D204)

Immunohistochemistry-Paraffin: Mouse Monoclonal Complement C4d Antibody (C4D204) [NBP2-34234]

Immunohistochemistry-Paraffin: Mouse Monoclonal Complement C4d Antibody (C4D204) [NBP2-34234] - Analysis of C4d on human lymph node tissue. Follicular dendritic cell are positive for C4d. pAb: 1:200 anti-mouse DAB epitope retrieval in basic buffer. Image from a verified customer review.

Applications for Complement C4d Antibody (C4D204)

Application
Recommended Usage

ELISA

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:200-1:400
Application Notes
ELISA: For coating use Ab at 1-2ug/ml, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1:200-1:400 for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34234 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement C4d

Alternate Names

C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, complement C4A, CPAMD2, RG

Gene Symbol

C4A

Additional Complement C4d Products

Product Documents for Complement C4d Antibody (C4D204)

Certificate of Analysis

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Product Specific Notices for Complement C4d Antibody (C4D204)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Complement C4d Antibody (C4D204)

Customer Reviews for Complement C4d Antibody (C4D204) (1)

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  • Complement C4d Antibody (C4D204)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lymph node
    Species: Human
    Verified Customer | Posted 02/05/2025
    follicular dendritic cell are positive for C4D
    pAb: 1:200 anti-mouse DAB epitope retrieval in basic buffer
    Complement C4d Antibody (C4D204) NBP2-34234

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