Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha-chain. Human C5a has four alpha-helices plus three intrachain disulfide bonds that create a triple loop structure.
Complement Component C5a Antibody (295103) [PerCP]
Novus Biologicals | Catalog # FAB2150C
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 295103
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Product Specifications
Immunogen
E. coli-derived recombinant mouse C5a
Asn679-Arg755
Accession # P06684
Asn679-Arg755
Accession # P06684
Specificity
Detects mouse Complement Component C5a in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross‑react with recombinant human (rh) C3a, rmC3d, or rhC5a.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Complement Component C5a Antibody (295103) [PerCP]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Complement Component C5a
Alternate Names
C5, C5A, HE
Gene Symbol
C5
Additional Complement Component C5a Products
Product Documents for Complement Component C5a Antibody (295103) [PerCP]
Certificate of Analysis
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Product Specific Notices for Complement Component C5a Antibody (295103) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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