Cortactin Antibody (23GB5130)

Novus Biologicals | Catalog # NBP3-50387

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5130
View all Cortactin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cortactin (Uniprot: Q14247)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cortactin Antibody (23GB5130)

Cortactin Antibody (23GB5130)

Flow Cytometry: Cortactin Antibody (23GB5130)[NBP3-50387] -

Flow cytometric analysis of Cortactinexpression in H9c2 cells using Cortactin antibody (1:2,000). Green, isotype control; red, Cortactin.
Cortactin Antibody (23GB5130)

Western Blot: Cortactin Antibody (23GB5130)[NBP3-50387] -

Western blotting analysis using Cortactin antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Cortactin Antibody (23GB5130)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cortactin

Immunocytochemistry reveals that in epithelial cells, the human cortactin protein is localized mainly in the cytoplasm and, to a very low extent, in protruding leading lamellae of the cell. However, in carcinoma cells that constitutively overexpress cortactin accumulates in the podosome-like adherens junctions associated with the cell-substratum contact sites. Overexpression and concomitant accumulation of the Cortactin protein in the cell-substratum contact sites might, therefore, contribute to the invasive potential of these tumor cells (1). In cells derived from breast carcinomas and squamous carcinomas of the head and neck, DNA amplification of a particular tyrosine kinase substrate region results in overexpression of cortactin. Overexpression is accompanied by a partial redistribution of cortactin from the cytoplasm into cell-matrix contact sites (2).

Alternate Names

Amplaxin, CTTN, EMS1

Gene Symbol

CTTN

Additional Cortactin Products

Product Documents for Cortactin Antibody (23GB5130)

Certificate of Analysis

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Product Specific Notices for Cortactin Antibody (23GB5130)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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