COS-7 Nuclear Hypoxic Induced Cell Lysate

Novus Biologicals | Catalog # NB800-PC26

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for COS-7 Nuclear Hypoxic Induced Cell Lysate

NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).

Product Specifications

Application Notes

Extracts have been quality control tested by Western blot. Before use mix 1:1 with 2X Sample Buffer and heat to 90C for 5 minutes before running on gel.

Specificity

NB800-PC26 contains hypoxia induced and uninduced COS-7 nuclear extract lysate

Type

Cell

Tissue

Digestive/Waste

Subcellular Fraction

Nuclear Hypoxic Induced

Scientific Data Images for COS-7 Nuclear Hypoxic Induced Cell Lysate

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).
Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26]

Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).

Formulation, Preparation, and Storage

Formulation

20 mM Hepes (pH 7.5), 400 mM NaCl, 0.1 mM EDTA, 10 mM NaF, 10 uM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors.

Preservative

20% glycerol

Concentration

2.5 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: COS-7

Cobalt Chloride (CoCl2) treated cells are often used as a positive control for HIF-1 and HIF-2 alpha analysis in hypoxia signaling experiments. HIFs induction can be conveniently achieved in vitro through CoCl2 treatment of cultured cells. CoCl2 inhibits prolyl-hydroxylases (PHD enzymes), the oxygen sensors, through replacement of Fe with Co making these enzymes unable to mark HIF alpha for degradation. In other words, CoCl2 allosterically blocks PHDs through its metal ion binding domain, hence, abolishing the VHL-HIF interaction which ultimately leads to the accumulation of HIFs in CoCl2 treated cells. CoCl2 has been regarded as one of the most reliable HIF-1 alpha inducer or hypoxia response mimicker, and this chemically induced hypoxia model is widely used in hypoxia-signaling research.

Additional COS-7 Products

Product Documents for COS-7 Nuclear Hypoxic Induced Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for COS-7 Nuclear Hypoxic Induced Cell Lysate

NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for COS-7 Nuclear Hypoxic Induced Cell Lysate

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FAQs for COS-7 Nuclear Hypoxic Induced Cell Lysate

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  • Q: I am interested in the above product with catalog number NB800-PC26 for western blot. Can I get more information about how the lysates are being produced before proceed with the decision of purchasing? It will be good if you have any protocols and reagents used particularly in western blot application with this product.

    A: NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. NB800-PC26 COS-7 nuclear extract was collected in Lysis Buffer after a 16-hour incubation with CoCl2 (0.15 mM). These cells come in 20 mM Hepes (pH 7.5), 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 uM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM beta-glycerophosphate, 1 mM DTT and protease inhibitors as a buffer.

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