COUP-TF II/NR2F2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31980

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Porcine

Predicted:

Bovine (100%), Chicken (100%), Human (100%), Rat (100%), Sheep (100%), Xenopus (100%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Knockdown Validated

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COUP-TF II/NR2F2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COUP-TF II/NR2F2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%). Porcine reactivity reported in scientific literature (PMID:31978970).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COUP-TF II/NR2F2 Antibody - BSA Free

Western Blot: COUP-TF II/NR2F2 Antibody [NBP1-31980]

Western Blot: COUP-TF II/NR2F2 Antibody [NBP1-31980]

Western Blot: COUP-TF II/NR2F2 Antibody [NBP1-31980] - Sample (20 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:10000
COUP-TF II/NR2F2 Antibody - BSA Free

Western Blot: COUP-TF II/NR2F2 Antibody - BSA Free [NBP1-31980] -

Effects of NR2F2 knockdown on progesterone biosynthesis. (A) gene expression of NR2F2 after siRNA transfection; (B) Effects of NR2F2 knockdown on progesterone secretion; (C) gene expression of key factors (StAR, Cyp11a1, HSD3B, and NPC1) involved in progesterone biosynthesis; (D,E) protein expression of NR5A2, StAR, Cyp11a1, and HSD3B. Primary pGCs were transfected with NR2F2 siRNA (siNR2F2) or scramble siRNA (siNC). Six hours later, the medium was changed to fresh medium with DMSO or DAPT, followed by further culture for 48 h. * means significant difference at p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31978970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for COUP-TF II/NR2F2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:5000-1:20000
Application Notes
Knockdown validation (PMID: 31978970).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COUP-TF II/NR2F2

COUP-TFII, a NR2 Hepatocyte NF4-Like receptor, has been shown to affect neuronal development and differentiation, homeostasis in various human tissues, mesenchymal-epithelial interactions during organogenesis, and the development of the cardiovascular system. COUP-TFII interacts with and modulates several nuclear receptors, including estrogen receptor alpha (ER alpha), thyroid hormone receptors (TR), PPAR alpha, and retinoic acid receptors (RAR). Mice lacking COUP-TFII show abnormal heart and vascular development. COUP-TFII expression has been documented in human adrenal, lung, kidney, liver, spleen, skeletal muscle, and breast. ESTs have been isolated from human tissue libraries, including cancerous adrenal, bladder, brain, breast, colon, head/neck, kidney, lung, ovary, pancreas, pituitary, prostate, skeletal muscle, skin, smooth muscle, stomach, synovium, and uterus, and normal brain, adipose, adrenal, blood, brain, breast, cervix, ear, embryo, gallbladder, ganglion, heart, kidney, liver/spleen, lung, ovary, placenta, prostate, skin, spleen, testis, uterus, and vessel.

Long Name

COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 2

Alternate Names

COUPTF II, NR2F2, SVP40

Gene Symbol

NR2F2

Additional COUP-TF II/NR2F2 Products

Product Documents for COUP-TF II/NR2F2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COUP-TF II/NR2F2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for COUP-TF II/NR2F2 Antibody - BSA Free

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