COX5A Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18416
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Chimpanzee (100%), Monkey (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recognizes region between residues Between Between 100 and 150.
Reactivity Notes
Gorilla (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COX5A Antibody - BSA Free
Western Blot-COX5A Antibody-NBP3-18416-
Western Blot-COX5A Antibody-NBP3-18416-Detection of human and mouse COX5A by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COX5A antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation-COX5A Antibody-NBP3-18416-
Immunoprecipitation-COX5A Antibody-NBP3-18416-Detection of human COX5A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5A antibody used for IP at 2 ug per reaction. COX5A was also immunoprecipitated by rabbit anti-COX5A antibody. For blotting immunoprecipitated COX5A, was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for COX5A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
20 ul/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: COX5A
Alternate Names
COX, COX-VA, Cytochrome c oxidase polypeptide Va, cytochrome c oxidase polypeptide, mitochondrial, cytochrome c oxidase subunit 5A, mitochondrial, cytochrome c oxidase subunit Va, mitochondrial cytochrome c oxidase subunit Va, VA
Gene Symbol
COX5A
Additional COX5A Products
Product Documents for COX5A Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for COX5A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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