CPSF6 Antibody (3F11) - Azide and BSA Free

Novus Biologicals | Catalog # H00011052-M10

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F11

Format

Azide and BSA Free
View all CPSF6 Primary Antibodies »

Product Specifications

Immunogen

CPSF6 (NP_008938, 37 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEAS

Specificity

CPSF6 (3F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CPSF6 Antibody (3F11) - Azide and BSA Free

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody (3F11) [H00011052-M10]

Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody (3F11) [H00011052-M10]

Immunocytochemistry/Immunofluorescence: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of monoclonal antibody to CPSF6 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in HeLa (Cat # L013V1).
Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in HL-60 (Cat # L014V1).
Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10]

Western Blot: CPSF6 Antibody (3F11) [H00011052-M10] - Analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody (M10), clone 3F11. Lane 1: CPSF6 transfected lysatE (59.21 KDa). Lane 2: Non-transfected lysate.
ELISA: CPSF6 Antibody (3F11) [H00011052-M10]

ELISA: CPSF6 Antibody (3F11) [H00011052-M10]

ELISA: CPSF6 Antibody (3F11) [H00011052-M10] - Detection limit for recombinant GST tagged CPSF6 is approximately 0.03ng/ml as a capture antibody.

Applications for CPSF6 Antibody (3F11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CPSF6

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Alternate Names

CFIM, CFIm68, CFIM68CPSF 68 kDa subunit, CFIMcleavage and polyadenylation specific factor 6, 68kD subunit, cleavage and polyadenylation specific factor 6, 68kDa, Cleavage and polyadenylation specificity factor 68 kDa subunit, cleavage and polyadenylation specificity factor subunit 6, HPBRII-4, HPBRII-7, pre-mRNA cleavage factor I, 68kD subunit, pre-mRNA cleavage factor Im (68kD), Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7

Entrez Gene IDs

11052 (Human)

Gene Symbol

CPSF6

OMIM

604979 (Human)

UniProt

NP_008938 (Human)

Additional CPSF6 Products

Product Documents for CPSF6 Antibody (3F11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CPSF6 Antibody (3F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CPSF6 Antibody (3F11) - Azide and BSA Free

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Protocols

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FAQs

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