Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CPSF6 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CPSF6.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CPSF6 Antibody - Azide and BSA Free

Western Blot: CPSF6 Antibody [NBP2-99189]

Western Blot: CPSF6 Antibody [NBP2-99189]

Western Blot: CPSF6 Antibody [NBP2-99189] - Anti-CPSF6 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]

Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]

Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189] - Immunochemical staining of human CPSF6 in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]

Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]

Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189] - Immunochemical staining of human CPSF6 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: CPSF6 Antibody [NBP2-99189]

Immunoprecipitation: CPSF6 Antibody [NBP2-99189]

Immunoprecipitation: CPSF6 Antibody [NBP2-99189] - CPSF6 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. Lane C: 0.5 mg Jurkat Whole Cell Lysate4 ul anti-CPSF6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CPSF6 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 72 kDa
CPSF6 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CPSF6 Antibody [NBP2-99189] -

Immunocytochemistry/Immunofluorescence: CPSF6 Antibody [NBP2-99189] - Immunofluorescence staining of CPSF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99189 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

Applications for CPSF6 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CPSF6

Alternate Names

CFIM, CFIm68, CFIM68CPSF 68 kDa subunit, CFIMcleavage and polyadenylation specific factor 6, 68kD subunit, cleavage and polyadenylation specific factor 6, 68kDa, Cleavage and polyadenylation specificity factor 68 kDa subunit, cleavage and polyadenylation specificity factor subunit 6, HPBRII-4, HPBRII-7, pre-mRNA cleavage factor I, 68kD subunit, pre-mRNA cleavage factor Im (68kD), Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7

Gene Symbol

CPSF6

Additional CPSF6 Products

Product Documents for CPSF6 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CPSF6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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