CRBN-6-5-5-VHL

Tocris Bioscience | Catalog # 6948

Potent and selective cereblon Degrader (PROTAC®); cell-permeable
Tocris Bioscience

Key Product Details

Description

Potent and selective cereblon Degrader (PROTAC®); cell-permeable
View all E3 Ligase Degraders »

Product Description

CRBN-6-5-5-VHL is a potent and selective cereblon Degrader (PROTAC®) (DC50 = 1.5 nM). Induces complete degradation of cereblon in MM1S cells. Comprises a cereblon E3 ligase ligand joined by a linker to a ligand for the E3 ligase VHL. Cell-permeable.

CRBN antibody validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # NBP1-91810.

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Scientific Data Images for CRBN-6-5-5-VHL

Application of CRBN-6-5-5-VHL in MM1S cells for CRBN-6-5-5-VHL

Application of CRBN-6-5-5-VHL in MM1S cells for CRBN-6-5-5-VHL.

Western blot data showing potent knockdown of Cereblon (CRBN) mediated by the Degrader: CRBN-6-5-5-VHL in MM1S cells after 16 h incubation. IKZF1 (Ikaros) and IKZF3 (Aiolos) are transcription factors and neosubstrates of Cereblon. Application of CRBN-6-5-5-VHL does not result in degradation of these proteins. Pom; Pomalidomide. Data courtesy of Christian Steinebach, University of Bonn.

Product Specifications for CRBN-6-5-5-VHL

Molecular Weight

972.21

Formula

C51H69N7O10S

Storage

Store at -20°C

Purity

≥96% (HPLC)

Chemical Name

(2S,4R)-1-((2S)-2-(5-((5-((6-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)hexyl)oxy)pentyl)oxy)pentanamido)-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide

CAS Number

2362575-45-7

SMILES

CC1=C(SC=N1)C2=CC=C(CNC([C@@H]3C[C@@H](O)CN3C([C@@H](NC(CCCCOCCCCCOCCCCCCNC4=CC=CC(C(N5C6CCC(NC6=O)=O)=O)=C4C5=O)=O)C(C)(C)C)=O)=O)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 97.22 100

Preparing Stock Solutions for CRBN-6-5-5-VHL

The following data is based on the product molecular weight 972.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.03 mL 5.14 mL 10.29 mL
5 mM 0.21 mL 1.03 mL 2.06 mL
10 mM 0.10 mL 0.51 mL 1.03 mL
50 mM 0.02 mL 0.10 mL 0.21 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for CRBN-6-5-5-VHL

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CRBN-6-5-5-VHL

For research use only

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