CRBN Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91810

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated, Single Cell Western

Cited:

Knockout Validated, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRBN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQERE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRBN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CRBN Antibody [NBP1-91810]

Immunocytochemistry/ Immunofluorescence: CRBN Antibody [NBP1-91810]

Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.
Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Simple Western: CRBN Antibody [NBP1-91810]

Simple Western: CRBN Antibody [NBP1-91810]

Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: CRBN Antibody [NBP1-91810]

Simple Western: CRBN Antibody [NBP1-91810]

Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).
Single Cell Western: CRBN Antibody [NBP1-91810]

Single Cell Western: CRBN Antibody [NBP1-91810]

Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.
Knockdown Validated: CRBN Antibody [NBP1-91810]

Western Blot: CRBN Antibody [NBP1-91810]

CRBN-Antibody-Western-Blot-NBP1-91810-img0036.jpg
CRBN Antibody - BSA Free Western Blot: CRBN Antibody - BSA Free [NBP1-91810]

Western Blot: CRBN Antibody - BSA Free [NBP1-91810]

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
CRBN Antibody - BSA Free Western Blot: CRBN Antibody - BSA Free [NBP1-91810]

Western Blot: CRBN Antibody - BSA Free [NBP1-91810]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for CRBN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Knockout Validated

Validation (PMID: 31371704).

Simple Western

1:60

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT4, U-251MG, separated by Size, antibody dilution of 1:60, apparent MW was 60 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 4 using NBP1-91810 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRBN

Modulates cell surface expression of KCNT1. May be involved in memory and learning

Long Name

Cereblon

Alternate Names

Cereblon, MRT2, MRT2A, Protein X 0001

Entrez Gene IDs

51185 (Human)

Gene Symbol

CRBN

UniProt

Q96SW2

Additional CRBN Products

Product Documents for CRBN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRBN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CRBN Antibody - BSA Free

Customer Reviews for CRBN Antibody - BSA Free (1)

4 out of 5
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Customer Images

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  • CRBN Antibody
    Name: Anonymous
    Application: In Cell Western
    Sample Tested: Cancer Cells
    Species: Human
    Verified Customer | Posted 11/14/2019
    CRBN expression after Downregulation by siRNA
    1/500 dilution
    CRBN Antibody - BSA Free NBP1-91810

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Protocols

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FAQs for CRBN Antibody - BSA Free

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  • Q: For the picture on your website, you run four cell lines. The lane 2 and 3 all have two bands. Which is the CRBN?

    A: Human CRBN has two known isoforms, both of which are approximately the same molecular weight, 50kDa. Given this information, it would be extremely difficult to distinguish the two in a WB. In our testing of the two cell lines, we did get multiple bands very close to each other, while in other lines or tissue lysates, we get one clean band. Unfortunately, we have not yet fully investigated the multiple banding pattern shown in the image. The antibody is polyclonal, so we typically tend to see some non-specific banding that can be cleaned up with further protocol optimization.

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