CRISPR-Cas13a Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB10463AFP680

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2608C
View all CRISPR-Cas13a Primary Antibodies »

Product Specifications

Specificity

Detects CRISPR-Cas13a in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for CRISPR-Cas13a Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CRISPR-Cas13a

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Prevotella and Francisella 1, Cpf1, also known as Cas12a, is a  1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system (1, 2), an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing (3). CRISPR/Cpf1 belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector (4). Cpf1 has recently emerged as an alternative for Cas9, due to its distinct features (2, 5) such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cpf1 is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs (6) which allows the possibility to use both mature or pre-crRNA for genome editing purposes(7). All these features make the CRISPR-Cpf1 system a valuable genome-engineering tool (8). CRISPR-Cpf1(Cas12a) has been successfully used to edit genomes in mammalians cells (2), plants (9), mice (10), Drosophila (11) and recently zebrafish and Xenopus (7). Two Cpf1 orthologs have been commonly used for genome editing in different organisms: AsCpf1 and LbCpf1, which are derived from Acidaminococcus sp. BV3L6 and Lachnospiraceae bacterium ND2006, respectively (8).The attached nuclear localization signals (NLSs) on the chimeric protein ensures nuclear compartmentalization in cells during gene editing (12).

References

  1. Jinek, M. et al. (2012) Science 337:816.
  2. Zetsche, B. et al. (2015) Cell 163:759.
  3. Sandler J.D. and Joung J.K. (2014) Nat Biotechnol. 32:347.
  4. Koonin E.V. et al. (2017) Curr. Opin. Micrbiol. 37:67.
  5. Bayat H. et al. (2018) Curr. Micribiol. 75:107.
  6. Fonfara, I. et al. (2016) Nature 532:517.
  7. Moreno-Mateo, M.A. et al. (2017) Nat. Commun 8:2024.
  8. Joung K.J. et al. (2016) Nat. Biotechnol. 34:869.
  9. Kim, H et al. (2017) Nat. Commun. 8:14406.
  10. Kim, Y. et al. (2016) Nat. Biotechnol. 34:808.
  11. Port, F. and Bullock, S.L. (2016) Nat Methods 13:852.
  12. Cong, L. et al. (2013) Science 339:819.

Long Name

CRISPR-associated Endoribonuclease 13a

UniProt

WP_003034647

Additional CRISPR-Cas13a Products

Product Documents for CRISPR-Cas13a Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for CRISPR-Cas13a Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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