Crk Antibody (M332)

Novus Biologicals | Catalog # NBP3-23251

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M332
View all Crk Primary Antibodies »

Product Specifications

Immunogen

Clone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II. [UniProt# Q64010]

Specificity

This antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk on SDS-PAGE immunoblots of rat PC12 and human Jurkat cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Crk Antibody (M332)

Crk Antibody (M332)

Western Blot: Mouse Crk mAb (M332) [NBP3-23251] -

Western Blot: Mouse Crk mAb (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).
Crk Antibody (M332)

Western Blot: Crk Antibody (M332) [NBP3-23251] -

Western Blot: Crk Antibody (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Applications for Crk Antibody (M332)

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Crk

Crk belongs to a family of adaptor molecules, which link extracellular growth and differentiation stimuli with formation of signal transduction protein complexes. Crk is mainly composed of Src homology 2 (SH2) and Src homology 3 (SH3) domains, which serve as docking sites for signalling proteins, including various receptors, cytoplasmic kinases, and GTPase regulators. These signal transduction pathways lead to modulation of cell adhesion, cell migration and immune cell responses.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog

Alternate Names

adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)

Gene Symbol

CRK

Additional Crk Products

Product Documents for Crk Antibody (M332)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Crk Antibody (M332)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins
PDGF Family

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