Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CSE1L/CAS Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Chromosome Segregation-1 using the numbering given in entry NP_001307.2 (GeneID 1434).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CSE1L/CAS/Exportin-2 Antibody - BSA Free (NB100-79803) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CSE1L/CAS/Exportin-2 Antibody - BSA Free

Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Human testicular seminoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (R; 50 mcg) cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CSE1L/CAS/Exportin-2 antibody BL1975 (Cat. No. NB100-79803) used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for WB and at 3 mcg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 3 minutes (A and B).
Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803]

Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of human CSE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 (lot NB100-79803-2) used for IP at 6 ug per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody (NB100-79803-1) and another rabbit anti-CSE1 antibody. For blotting immunoprecipitated CSE1, NB100-79803 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CSE1L/CAS/Exportin-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000-1:10000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:10000-1:25000
Application Notes
Immunohistochemistry 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CSE1L/CAS

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation.

Long Name

Chromosome segregation 1-like protein

Alternate Names

CAS, CSE1, XPO2

Entrez Gene IDs

1434 (Human)

Gene Symbol

CSE1L

UniProt

P55060 (Human)

Additional CSE1L/CAS Products

Product Documents for CSE1L/CAS/Exportin-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CSE1L/CAS/Exportin-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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