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MycoProbe Mycoplasma Detection Kit

Catalog #: CUL001B
107 Citations 6 Reviews Datasheet / COA / SDS
Mycoplasmas Detected: M. hyorhinis, M. arginini, M. fermentans, M. orale, M. pirum, M. hominis, M. salivarium, Acholeplasma laidlawii
Catalog # Availability Size / Price Qty
CUL001B

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Mycoplasma Detection in Cell Line Supernates and Cell Lysates. 
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Product Details
Procedure
Citations (107)
FAQs
Reviews (6)
Summary Product Datasheets Data Images

MycoProbe Mycoplasma Detection Kit Summary

A complete kit to detect common antibiotic-resistant cell culture contaminants

Key Benefits

  • Reduces experimental variation
  • Accurate and highly sensitive
  • Simple multiwell-based colorimetric assay
  • Only takes 4.5 hours

 

Why should I be worried about mycoplasma contamination?

Mycoplasma contamination affects up to 80% of continuous cell cultures. If undetected, mycoplasma contamination can have significant effects on the quality and reliability of your cell culture preparations.

Mycoplasma contamination:

  • Induces deleterious effects on cell culture quality.
  • Alters the phenotypic characteristics of host cells.
  • Increases experimental variation.
  • Is common in eukaryotic cell cultures.
  • Is resistant to antibiotics such as penicillin and streptomycin.
  • Has multiple sources (i.e. personnel, reagents, other infected cells).

Mycoplasma are difficult to detect because they:

  • Are not visible using standard microscopes.
  • Are small enough to pass through 0.45 mm sterilization filters.
  • Do not produce changes in culture medium color, pH, or turbidit

What is the best method to determine if my cultured cells are mycoplasma-contaminated?

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA) using a colorimetric signal amplification system with sensitivity comparable to PCR. The MycoProbe assay is not susceptible to common problems encountered with PCR-based mycoplasma detection kits.

The MycoProbe Mycoplasma Detection Assay:

  • Detects the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
  • Is highly sensitive (comparable to PCR).
  • Is compatible with high-throughput screening.
  • Does not generate false positives from amplicon contamination.
  • Can be used for cell culture supernatants or cultured cell pellets.
  • Can be used with samples from fresh or frozen cells.
  • Does not require cells to be cultured in antibiotic-free media.
  • Includes a synthetic DNA oligonucleotide positive control.
  • Generates results in 4.5 hours.

Alternative Methods for Mycoplasma Detection

Technique

Advantage

Disadvantage
Microbiological culture Most sensitive
  • Takes 2 to 4 weeks
  • Cannot detect fastidious mycoplasma
  • Requires specialized laboratory conditions
Fluorescent DNA staining Efficient
  • Cannot detect mycoplasma that cyto-absorb poorly
ELISA of cell surface antigens Common technique
  • Low sensitivity
PCR-based detection High sensitivity
  • Prone to both false positives and false negatives
Biochemical activity Common technique
  • Inconsistent results across cell lines
Kit Components

The MycoProbeTM Mycoplasma Detection Kit (Catalog # CUL001B) contains enough reagents to assay one 96-well plate for mycoplasma contamination.

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips
Precautions

The Wash Buffer supplied in this kit contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

When handling cell culture samples, appropriate precautions should be taken to prevent exposure to mycoplasma and other hazardous biological agents.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Data Example

Mycoplasma Detection Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  View Larger

Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  The presence of the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination was tested in supernates and cell lysates of the indicated cell lines using the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B). The average of the duplicate optical density (OD) readings for each control and sample was determined. The average negative control OD value was subtracted from all average sample OD values. A calculated positive control OD value of >0.10 indicated mycoplasma contamination (black line) in the CTLL-2, BaF3, A431, and K562 (cell lysate 1) samples. Abbreviations: CTLL-2 mouse cytotoxic T cell line, BaF3 mouse pro-B cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mycoplasma contamination can be evaluated in cell culture supernates or cell pellets using this straightforward procedure:

  • Samples are lysed and hybridized with biotin-labeled capture oligonucleotide probes
  • Digoxigenin-labeled detection probes target the eight most common mycoplasma contaminants
  • Following signal amplification, multiwells are measured using a standard colorimetric plate reader
  • Results are generated in 4.5 hours
CUL001B Protocol Graphic Summary
View Graphic Procedure
 

 

Reagents Provided

Reagents supplied in the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B):

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips

 

Other Supplies Required

Reagents

  • Deionized water, RNase-free
  • Cell samples of interest

Materials

  • Pipettes and pipette tips
  • Squirt bottle or manifold dispenser
  • 100 mL and 1,000 mL graduated cylinders for preparation of Wash Buffer
  • Gloves and mask

Equipment

  • Microplate reader capable of measuring absorbance at 490 nm with the correction wavelength set at 650 nm or 690 nm
  • Horizontal orbital microplate shaker (0.12" orbit) capable of maintaining a speed of 500 + 50 rpm
  • 65 + 1 °C water bath
  • Vortex mixer

 

Procedure Overview

R&D Systems Protocol for Mouse Treg Cell Differentiation

Wash the Hybridization Plate 2 times with Wash Buffer.

Add diluted Probes, Positive Control, Sample Diluent (Negative Control), or sample to the designated wells.

Incubate the plate for 60 minutes in a 65 °C water bath.

Protocol for Mouse Treg Cell Differentiation Step 1

Wash the Streptavidin Plate 2 times with Wash Buffer.

Transfer 150 µL from each well of the Hybridization Plate to the Streptavidin Plate.

Incubate for 60 minutes on a horizontal orbital shaker.

Protocol for Mouse Treg Cell Differentiation Step 2

Wash the Streptavidin Plate 4 times with Wash Buffer.

Add Anti-Digoxigenin Conjugate to each well.

Incubate for 60 minutes on a shaker.

Protocol for Mouse Treg Cell Differentiation Step 3

Wash the Streptavidin Plate 6 times with Wash Buffer.

Add Substrate Solution to each well.

Incubate for 60 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 4

Add Amplifier Solution to each well.

Incubate for 30 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 5

Add Stop Solution to each well.

Determine the optical density (OD) of each well within 30 minutes, using a microplate reader set to 490 nm.

Protocol for Mouse Treg Cell Differentiation Step 6

Note: If wavelength correction is available, set to 650 nm or 690 nm. If wavelength correction is not available, subtract readings at 650 nm or 690 nm from the readings at 490 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 490 nm without correction may be higher and less accurate.

 
 
Calculation of Results

Determine the average of the duplicate optical density (OD) readings for each control and sample. Subtract the average negative control OD value from all average OD values. The calculated positive control OD value should be > 1.5.

OD Values (Calculated)

Result

Interpretation
< 0.05 Negative No mycoplasma detected
0.05 – 0.10 Inconclusive Sample is suspect for mycoplasma. Continue to culture for an additional 2 - 3 days and repeat the test. If sample gives a similar OD, then no mycoplasma are detected.
> 0.10 Positive Mycoplasma detected

Citations for MycoProbe Mycoplasma Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

107 Citations: Showing 1 - 10
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  1. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022;0(0):102029.  2022
  2. Molecular basis of crosstalk in nuclear receptors: heterodimerization between PXR and CAR and the implication in gene regulation
    Authors: MN Bwayi, E Garcia-Mal, SC Chai, B Xie, S Chodankar, AD Huber, J Wu, K Annu, WC Wright, HM Lee, J Seetharama, J Wang, CD Buchman, J Peng, T Chen
    Nucleic Acids Research, 2022;0(0):.  2022
  3. [89Zr]ZrDFO-CR011 PET Correlates with Response to Glycoprotein Nonmetastatic Melanoma B-targeted Therapy in Triple-negative Breast Cancer
    Authors: S Lee, A Cavaliere, JD Gallezot, T Keler, SK Michelhaug, E Belitzky, M Liu, T Mulnix, SE Maher, ALM Bothwell, F Li, M Phadke, S Mittal, B Marquez-No
    Molecular Cancer Therapeutics, 2022;21(3):440-447.  2022
  4. Combining p53 mRNA nanotherapy with immune checkpoint blockade reprograms the immune microenvironment for effective cancer therapy
    Authors: Y Xiao, J Chen, H Zhou, X Zeng, Z Ruan, Z Pu, X Jiang, A Matsui, L Zhu, Z Amoozgar, DS Chen, X Han, DG Duda, J Shi
    Nature Communications, 2022;13(1):758.  2022
  5. Galvanic current activates the NLRP3 inflammasome to promote Type I collagen production in tendon
    Authors: A Peñin-Fran, JA García-Vid, CM Martínez, P Escolar-Re, RM Martínez-O, AI Gómez, JM Bueno, F Minaya-Muñ, F Valera-Gar, F Medina-Mir, P Pelegrín
    Elife, 2022;11(0):.  2022
  6. Evolutionary analyses of the gasdermin family suggest conserved roles in infection response despite loss of pore-forming functionality
    Authors: D Angosto-Ba, C Alarcón-Vi, L Hurtado-Na, MC Baños, J Rivers-Aut, P Pelegrín
    Bmc Biology, 2022;20(1):9.  2022
  7. Autophagy-competent mitochondrial translation elongation factor TUFM inhibits caspase-8-mediated apoptosis
    Authors: CY Choi, MT Vo, J Nicholas, YB Choi
    Cell Death and Differentiation, 2021;0(0):.  2021
  8. Mesenchymal stem cell-derived interleukin-28 drives the selection of apoptosis resistant bone metastatic prostate cancer
    Authors: JJ McGuire, JS Frieling, CH Lo, T Li, A Muhammad, HR Lawrence, NJ Lawrence, LM Cook, CC Lynch
    Nature Communications, 2021;12(1):723.  2021
  9. Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancers
    Authors: N Mao, Z Zhang, YS Lee, D Choi, AA Rivera, D Li, C Lee, S Haywood, X Chen, Q Chang, G Xu, HA Chen, E de Stanchi, C Sawyers, N Rosen, AC Hsieh, Y Chen, BS Carver
    Nature Communications, 2021;12(1):5053.  2021
  10. Liquid biopsy genotyping by a simple lateral flow strip assay with visual detection
    Authors: P Kalligosfy, S Nikou, V Bravou, DP Kalogianni
    Analytica chimica acta, 2021;1163(0):338470.  2021
  11. Long-Chain Acylcarnitines Decrease the Phosphorylation of the Insulin Receptor at Tyr1151 Through a PTP1B-Dependent Mechanism
    Authors: K Vilks, M Videja, M Makrecka-K, M Katkevics, E Sevostjano, A Grandane, M Dambrova, E Liepinsh
    International Journal of Molecular Sciences, 2021;22(12):.  2021
  12. Mutant APC promotes tumor immune evasion via PD-L1 in colorectal cancer
    Authors: B Cen, J Wei, D Wang, Y Xiong, JW Shay, RN DuBois
    Oncogene, 2021;0(0):.  2021
  13. MAOA promotes prostate cancer cell perineural invasion through SEMA3C/PlexinA2/NRP1-cMET signaling
    Authors: L Yin, J Li, J Wang, T Pu, J Wei, Q Li, BJ Wu
    Oncogene, 2021;0(0):.  2021
  14. A novel strategy for combination of clofarabine and pictilisib is synergistic in gastric cancer
    Authors: S Khalafi, S Zhu, R Khurana, I Lohse, S Giordano, S Corso, H Al-Ali, SP Brothers, C Wahlestedt, S Schürer, W El-Rifai
    Translational Oncology, 2021;15(1):101260.  2021
  15. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer
    Authors: Z Wang, Y Goto, MM Allevato, VH Wu, R Saddawi-Ko, M Gilardi, D Alvarado, BS Yung, A O'Farrell, AA Molinolo, U Duvvuri, JR Grandis, JA Califano, EEW Cohen, JS Gutkind
    Nature Communications, 2021;12(1):2383.  2021
  16. The Angiotensin II Receptor Blocker Losartan Sensitizes Human Liver Cancer Cells to Lenvatinib-Mediated Cytostatic and Angiostatic Effects
    Authors: H Takagi, K Kaji, N Nishimura, K Ishida, H Ogawa, H Takaya, H Kawaratani, K Moriya, T Namisaki, T Akahane, A Mitoro, H Yoshiji
    Cells, 2021;10(3):.  2021
  17. Intravital molecular imaging reveals the restrained capacity of CTLs in the killing of tumor cells in the liver
    Authors: L Liu, B Dai, R Li, Z Liu, Z Zhang
    Theranostics, 2021;11(1):194-208.  2021
  18. Exosomal miR-1260b derived from non-small cell lung cancer promotes tumor metastasis through the inhibition of HIPK2
    Authors: DH Kim, H Park, YJ Choi, MH Kang, TK Kim, CG Pack, CM Choi, JC Lee, JK Rho
    Cell Death & Disease, 2021;12(8):747.  2021
  19. NAD+ boosting reduces age-associated amyloidosis and restores mitochondrial homeostasis in muscle
    Authors: M Romani, V Sorrentino, CM Oh, H Li, TI de Lima, H Zhang, M Shong, J Auwerx
    Cell Reports, 2021;34(3):108660.  2021
  20. Tumor-conditional IL-15 pro-cytokine reactivates anti-tumor immunity with limited toxicity
    Authors: J Guo, Y Liang, D Xue, J Shen, Y Cai, J Zhu, YX Fu, H Peng
    Cell Research, 2021;0(0):.  2021
  21. A Novel Monoclonal Antibody Targeting Cancer-Specific Plectin Has Potent Antitumor Activity in Ovarian Cancer
    Authors: SM Perez, J Dimastroma, CN Landen, KA Kelly
    Cells, 2021;10(9):.  2021
  22. Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2
    Authors: Y Gu, J Cao, X Zhang, H Gao, Y Wang, J Wang, J He, X Jiang, J Zhang, G Shen, J Yang, X Zheng, G Hu, Y Zhu, S Du, Y Zhu, R Zhang, J Xu, F Lan, D Qu, G Xu, Y Zhao, D Gao, Y Xie, M Luo, Z Lu
    Cell Research, 2021;0(0):.  2021
  23. Cooling-mediated protection from chemotherapy drug-induced cytotoxicity in human keratinocytes by inhibition of cellular drug uptake
    Authors: C Dunnill, K Ibraheem, M Peake, M Ioannou, M Palmer, A Smith, A Collett, NT Georgopoul
    PLoS ONE, 2020;15(10):e0240454.  2020
  24. CD73 blockade enhances the local and abscopal effects of radiotherapy in a murine rectal cancer model
    Authors: H Tsukui, H Horie, K Koinuma, H Ohzawa, Y Sakuma, Y Hosoya, H Yamaguchi, K Yoshimura, AK Lefor, N Sata, J Kitayama
    BMC Cancer, 2020;20(1):411.  2020
  25. Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity
    Authors: HL Mercer, LM Snyder, CM Doherty, BA Fox, DJ Bzik, EY Denkers
    PLoS Pathog., 2020;16(5):e1008572.  2020
  26. MAFB promotes cancer stemness and tumorigenesis in osteosarcoma through a Sox9-mediated positive feedback loop
    Authors: Y Chen, B Wang, M Huang, T Wang, C Hu, Q Liu, D Han, C Chen, J Zhang, Z Li, C Liu, W Lei, Y Chang, M Wu, D Xiang, Y Chen, R Wang, W Huang, Z Lei, X Chu
    Cancer Res., 2020;0(0):.  2020
  27. EIF3H orchestrates Hippo pathway-mediated oncogenesis via catalytic control of YAP stability
    Authors: Z Zhou, H Zhou, L Ponzoni, A Luo, R Zhu, M He, Y Huang, KL Guan, I Bahar, Z Liu, Y Wan
    Cancer Res., 2020;0(0):.  2020
  28. Comprehensive RNA-Seq profiling of the lung transcriptome of Bashbay sheep in response to experimental Mycoplasma ovipneumoniae infection
    Authors: Z Du, Y Sun, J Wang, H Liu, Y Yang, N Zhao
    PLoS ONE, 2020;15(7):e0214497.  2020
  29. KANSL2 and MBNL3 are regulators of pancreatic ductal adenocarcinoma invasion
    Authors: PO Oladimeji, J Bakke, WC Wright, T Chen
    Sci Rep, 2020;10(1):1485.  2020
  30. &alpha-Linolenic acid but not linolenic acid protects against hypertension: critical role of SIRT3 and autophagic flux
    Authors: G Li, X Wang, H Yang, P Zhang, F Wu, Y Li, Y Zhou, X Zhang, H Ma, W Zhang, J Li
    Cell Death Dis, 2020;11(2):83.  2020
  31. Oncogenic ERG represses PI3K signaling through down-regulation of IRS2
    Authors: N Mao, D Gao, W Hu, S Gadal, H Hieronymus, S Wang, YS Lee, P Sullivan, Z Zhang, D Choi, N Rosen, CL Sawyers, A Gopalan, Y Chen, BS Carver
    Cancer Res., 2020;0(0):.  2020
  32. Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage
    Authors: X Fan, Z Hao, Z Li, X Wang, J Wang
    Mediators Inflamm., 2020;2020(0):6126490.  2020
  33. Maintenance Therapy for ATM-Deficient Pancreatic Cancer by Multiple DNA Damage Response Interferences after Platinum-Based Chemotherapy
    Authors: E Roger, J Gout, F Arnold, AK Beutel, M Müller, A Abaei, TFE Barth, V Rasche, T Seufferlei, L Perkhofer, A Kleger
    Cells, 2020;9(9):.  2020
  34. Epigenetic targeting of neuropilin-1 prevents bypass signaling in drug-resistant breast cancer
    Authors: A Abdullah, SS Akhand, JSP Paez, W Brown, L Pan, S Libring, M Badamy, E Dykuizen, L Solorio, W Andy Tao, MK Wendt
    Oncogene, 2020;0(0):.  2020
  35. Novel oral plasminogen activator inhibitor?1 inhibitor TM5275 attenuates hepatic fibrosis under metabolic syndrome via suppression of activated hepatic stellate cells in rats
    Authors: R Noguchi, K Kaji, T Namisaki, K Moriya, H Kawaratani, M Kitade, H Takaya, Y Aihara, A Douhara, K Asada, N Nishimura, T Miyata, H Yoshiji
    Molecular Medicine Reports, 2020;0(0):.  2020
  36. Redox homeostasis maintained by GPX4 facilitates STING activation
    Authors: M Jia, D Qin, C Zhao, L Chai, Z Yu, W Wang, L Tong, L Lv, Y Wang, J Rehwinkel, J Yu, W Zhao
    Nat. Immunol., 2020;21(7):727-735.  2020
  37. Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage
    Authors: X Fan, Z Hao, Z Li, X Wang, J Wang
    Mediators Inflamm., 2020;2020(0):6126490.  2020
  38. Neutrophil infiltration and whole-cell vaccine elicited by N-dihydrogalactochitosan combined with NIR phototherapy to enhance antitumor immune response and T cell immune memory
    Authors: S Qi, L Lu, F Zhou, Y Chen, M Xu, L Chen, X Yu, WR Chen, Z Zhang
    Theranostics, 2020;10(4):1814-1832.  2020
  39. Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies
    Authors: A Anloague, A Mahoney, O Ogunbekun, TA Hiland, WR Thompson, B Larsen, MT Loghmani, JM Hum, JW Lowery
    BMC Res Notes, 2020;13(1):400.  2020
  40. Evaluation of chemotherapy and P2Et extract combination in ex-vivo derived tumor mammospheres from breast cancer patients
    Authors: C Urueña, TA Sandoval, P Lasso, M Tawil, A Barreto, L Torregrosa, S Fiorentino
    Sci Rep, 2020;10(1):19639.  2020
  41. Expression of Tryptophan 2,3-Dioxygenase in Metastatic Uveal Melanoma
    Authors: M Terai, E Londin, A Rochani, E Link, B Lam, G Kaushal, A Bhushan, M Orloff, T Sato
    Cancers (Basel), 2020;12(2):.  2020
  42. BET, SRC, and BCL2 family inhibitors are synergistic drug combinations with PARP inhibitors in ovarian cancer
    Authors: GYL Lui, R Shaw, FX Schaub, IN Stork, KE Gurley, C Bridgwater, RL Diaz, R Rosati, HA Swan, TA Ince, TC Harding, VK Gadi, BA Goff, CJ Kemp, EM Swisher, C Grandori
    EBioMedicine, 2020;60(0):102988.  2020
  43. Loss of the transcription factor MAFB limits beta-cell derivation from human PSCs
    Authors: R Russell, PP Carnese, TG Hennings, EM Walker, HA Russ, JS Liu, S Giacometti, R Stein, M Hebrok
    Nat Commun, 2020;11(1):2742.  2020
  44. The ITIM-Containing Receptor: Leukocyte-Associated Immunoglobulin-Like Receptor-1 (LAIR-1) Modulates Immune Response and Confers Poor Prognosis in Invasive Breast Carcinoma
    Authors: C Joseph, MA Alsaleem, MS Toss, YA Kariri, M Althobiti, S Alsaeed, AI Aljohani, PL Narasimha, NP Mongan, AR Green, EA Rakha
    Cancers, 2020;13(1):.  2020
  45. Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells
    Authors: J Bakke, WC Wright, AE Zamora, P Oladimeji, JC Crawford, CT Brewer, RJ Autry, WE Evans, PG Thomas, T Chen
    BMC Cancer, 2019;19(1):253.  2019
  46. Influence of Vitamin D on Corneal Epithelial Cell Desmosomes and Hemidesmosomes
    Authors: X Lu, MA Watsky
    Invest. Ophthalmol. Vis. Sci., 2019;60(13):4074-4083.  2019
  47. Genome-wide miRNA profiling and pivotal roles of miRs 125a-5p and 17-92 cluster in human neutrophil maturation and differentiation of acute myeloid leukemia cells
    Authors: EH Dakir, F Mollinedo
    Oncotarget, 2019;10(51):5313-5331.  2019
  48. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson's Disease Model
    Authors: J Han, SJ Kim, MJ Ryu, Y Jang, MJ Lee, X Ju, YL Lee, J Cui, M Shong, JY Heo, GR Kweon
    Oxid Med Cell Longev, 2019;2019(0):4174803.  2019
  49. Investigation of Genetic Susceptibility to Blastomycosis Reveals Interleukin-6 as a Potential Susceptibility Locus
    Authors: RM Merkhofer, MB O'Neill, D Xiong, N Hernandez-, H Dobson, JS Fites, AC Shockey, M Wuethrich, CS Pepperell, BS Klein
    MBio, 2019;10(3):.  2019
  50. CD38-driven mitochondrial trafficking promotes bioenergetic plasticity in multiple myeloma
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    Cancer Res., 2019;0(0):.  2019
  51. Centrosome amplification in cancer disrupts autophagy and sensitizes to autophagy inhibition
    Authors: RA Denu, G Kaur, MM Sass, A Lakkaraju, ME Burkard
    Mol. Cancer Res., 2019;0(0):.  2019
  52. Delivery of mRNA vaccines with heterocyclic lipids increases anti-tumor efficacy by STING-mediated immune cell activation
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FAQs

  1. What are recommendations for cell culture numbers prior to evaluating supernatant in the kit?

    • For adherent cells, we recommend culturing cells to confluence prior to the assay. For suspension cells, we recommend culturing to a density of 0.5-1 x 106 cells/mL.

View all Cell Culture Product FAQs

Reviews for MycoProbe Mycoplasma Detection Kit

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MycoProbe Mycoplasma Detection Kit
By Vincent Giacalone on 05/20/2021

This is a alternative to PCR for mycoplasma testing. I used it to test cell culture supernatants but can also be used for cell lysates. The positive control wells are in red and negative control are in green. 14 samples were measured in duplicate and after subtracting the negative control all were well below the lower threshold of 0.05. The kit assay requires about 6 hours to complete but mostly consists of incubation steps, one of which requires 65 degree water bath. The one downside is the kit is best for measuring large batches of samples all at once given the plate based format.

MycoProbe Mycoplasma Detection Kit CUL001B
MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/05/2020

This is the best kit I have ever used for testing mycoplasma, better than PCR and fluorescence based assays. It can test both cells and media, both frozen and fresh samples. It does not require 2 weeks antibiotic free culture time. It takes about 5 to 6 hours to finish. The only not very convenient step is the 65C incubation in water bath.

MycoProbe Mycoplasma Detection Kit CUL001B
MycoProbe Mycoplasma Detection Kit
By Anonymous on 12/06/2019
MycoProbe Mycoplasma Detection Kit
By Leslie Priddy on 04/03/2018
MycoProbe Mycoplasma Detection Kit
By Anonymous on 07/07/2017
MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/13/2016

Our lab has been using this kit for routine Mycoplasma testing of cultures for over 2 years now. The kit is highly sensitive and specific to Mycoplasma. The data is reproducible and more accurate than other tests, the size of the assay is adjustable (the kit comes as 12 8-well strips). You get reliable results in a few hours. Overall, great product and highly recommended.

MycoProbe Mycoplasma Detection Kit CUL001B