MycoProbe Mycoplasma Detection Kit

Mycoplasmas Detected: M. hyorhinis, M. arginini, M. fermentans, M. orale, M. pirum, M. hominis, M. salivarium, Acholeplasma laidlawii
Catalog # Availability Size / Price Qty
CUL001B
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Mycoplasma Detection in Cell Line Supernates and Cell Lysates. 
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Product Details
Procedure
Citations (125)
FAQs
Reviews (6)

MycoProbe Mycoplasma Detection Kit Summary

A complete kit to detect common antibiotic-resistant cell culture contaminants

Key Benefits

  • Reduces experimental variation
  • Accurate and highly sensitive
  • Simple multiwell-based colorimetric assay
  • Only takes 4.5 hours

 

Why should I be worried about mycoplasma contamination?

Mycoplasma contamination affects up to 80% of continuous cell cultures. If undetected, mycoplasma contamination can have significant effects on the quality and reliability of your cell culture preparations.

Mycoplasma contamination:

  • Induces deleterious effects on cell culture quality.
  • Alters the phenotypic characteristics of host cells.
  • Increases experimental variation.
  • Is common in eukaryotic cell cultures.
  • Is resistant to antibiotics such as penicillin and streptomycin.
  • Has multiple sources (i.e. personnel, reagents, other infected cells).

Mycoplasma are difficult to detect because they:

  • Are not visible using standard microscopes.
  • Are small enough to pass through 0.45 mm sterilization filters.
  • Do not produce changes in culture medium color, pH, or turbidit

What is the best method to determine if my cultured cells are mycoplasma-contaminated?

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA) using a colorimetric signal amplification system with sensitivity comparable to PCR. The MycoProbe assay is not susceptible to common problems encountered with PCR-based mycoplasma detection kits.

The MycoProbe Mycoplasma Detection Assay:

  • Detects the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
  • Is highly sensitive (comparable to PCR).
  • Is compatible with high-throughput screening.
  • Does not generate false positives from amplicon contamination.
  • Can be used for cell culture supernatants or cultured cell pellets.
  • Can be used with samples from fresh or frozen cells.
  • Does not require cells to be cultured in antibiotic-free media.
  • Includes a synthetic DNA oligonucleotide positive control.
  • Generates results in 4.5 hours.

Alternative Methods for Mycoplasma Detection

Technique

Advantage

Disadvantage

Microbiological culture Most sensitive
  • Takes 2 to 4 weeks
  • Cannot detect fastidious mycoplasma
  • Requires specialized laboratory conditions
Fluorescent DNA staining Efficient
  • Cannot detect mycoplasma that cyto-absorb poorly
ELISA of cell surface antigens Common technique
  • Low sensitivity
PCR-based detection High sensitivity
  • Prone to both false positives and false negatives
Biochemical activity Common technique
  • Inconsistent results across cell lines
Kit Components

The MycoProbeTM Mycoplasma Detection Kit (Catalog # CUL001B) contains enough reagents to assay one 96-well plate for mycoplasma contamination.

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips
Precautions

The Wash Buffer supplied in this kit contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

When handling cell culture samples, appropriate precautions should be taken to prevent exposure to mycoplasma and other hazardous biological agents.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

Mycoplasma Detection Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  View Larger

Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  The presence of the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination was tested in supernates and cell lysates of the indicated cell lines using the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B). The average of the duplicate optical density (OD) readings for each control and sample was determined. The average negative control OD value was subtracted from all average sample OD values. A calculated positive control OD value of >0.10 indicated mycoplasma contamination (black line) in the CTLL-2, BaF3, A431, and K562 (cell lysate 1) samples. Abbreviations: CTLL-2 mouse cytotoxic T cell line, BaF3 mouse pro-B cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mycoplasma contamination can be evaluated in cell culture supernates or cell pellets using this straightforward procedure:

  • Samples are lysed and hybridized with biotin-labeled capture oligonucleotide probes
  • Digoxigenin-labeled detection probes target the eight most common mycoplasma contaminants
  • Following signal amplification, multiwells are measured using a standard colorimetric plate reader
  • Results are generated in 4.5 hours
 

 

Reagents Provided

Reagents supplied in the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B):

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips

 

Other Supplies Required

Reagents

  • Deionized water, RNase-free
  • Cell samples of interest

Materials

  • Pipettes and pipette tips
  • Squirt bottle or manifold dispenser
  • 100 mL and 1,000 mL graduated cylinders for preparation of Wash Buffer
  • Gloves and mask

Equipment

  • Microplate reader capable of measuring absorbance at 490 nm with the correction wavelength set at 650 nm or 690 nm
  • Horizontal orbital microplate shaker (0.12" orbit) capable of maintaining a speed of 500 + 50 rpm
  • 65 + 1 °C water bath
  • Vortex mixer

 

Procedure Overview

R&D Systems Protocol for Mouse Treg Cell Differentiation

Wash the Hybridization Plate 2 times with Wash Buffer.

Add diluted Probes, Positive Control, Sample Diluent (Negative Control), or sample to the designated wells.

Incubate the plate for 60 minutes in a 65 °C water bath.

Protocol for Mouse Treg Cell Differentiation Step 1

Wash the Streptavidin Plate 2 times with Wash Buffer.

Transfer 150 µL from each well of the Hybridization Plate to the Streptavidin Plate.

Incubate for 60 minutes on a horizontal orbital shaker.

Protocol for Mouse Treg Cell Differentiation Step 2

Wash the Streptavidin Plate 4 times with Wash Buffer.

Add Anti-Digoxigenin Conjugate to each well.

Incubate for 60 minutes on a shaker.

Protocol for Mouse Treg Cell Differentiation Step 3

Wash the Streptavidin Plate 6 times with Wash Buffer.

Add Substrate Solution to each well.

Incubate for 60 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 4

Add Amplifier Solution to each well.

Incubate for 30 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 5

Add Stop Solution to each well.

Determine the optical density (OD) of each well within 30 minutes, using a microplate reader set to 490 nm.

Protocol for Mouse Treg Cell Differentiation Step 6

Note: If wavelength correction is available, set to 650 nm or 690 nm. If wavelength correction is not available, subtract readings at 650 nm or 690 nm from the readings at 490 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 490 nm without correction may be higher and less accurate.

 
 
Calculation of Results

Determine the average of the duplicate optical density (OD) readings for each control and sample. Subtract the average negative control OD value from all average OD values. The calculated positive control OD value should be > 1.5.

OD Values (Calculated)

Result

Interpretation

< 0.05 Negative No mycoplasma detected
0.05 – 0.10 Inconclusive Sample is suspect for mycoplasma. Continue to culture for an additional 2 - 3 days and repeat the test. If sample gives a similar OD, then no mycoplasma are detected.
> 0.10 Positive Mycoplasma detected

Citations for MycoProbe Mycoplasma Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

125 Citations: Showing 1 - 10
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  1. Polypharmacology-based kinome screen identifies new regulators of KSHV reactivation
    Authors: Olson, AT;Kang, Y;Ladha, AM;Zhu, S;Lim, CB;Nabet, B;Lagunoff, M;Gujral, TS;Geballe, AP;
    PLoS pathogens  2023-09-01
  2. PTPN2 copper-sensing rapidly relays copper level fluctuations into EGFR/CREB activation and associated CTR1 transcriptional repression
    Authors: Ross, MO;Xie, Y;Owyang, RC;Ye, C;Zbihley, ONP;Lyu, R;Wu, T;Wang, P;Karginova, O;Olopade, OI;Zhao, M;He, C;
    bioRxiv : the preprint server for biology  2023-08-29
  3. A robust method for measuring aminoacylation through tRNA-Seq
    Authors: Davidsen, K;Sullivan, LB;
    bioRxiv : the preprint server for biology  2023-08-01
  4. Selenoprotein K enhances STING oligomerization to facilitate antiviral response
    Authors: L Lv, L Chai, J Wang, M Wang, D Qin, H Song, Y Fu, C Zhao, J Jia, W Zhao, M Jia
    PloS Pathogens, 2023-04-06;19(4):e1011314.  2023-04-06
  5. WNT5a Signaling through ROR2 Activates the Hippo Pathway to Suppress YAP1 Activity and Tumor Growth
    Authors: K Wang, F Ma, S Arai, Y Wang, A Varkaris, L Poluben, O Voznesensk, F Xie, X Zhang, X Yuan, SP Balk
    Cancer Research, 2023-04-04;0(0):.  2023-04-04
  6. A rare human variant that disrupts GPR10 signalling causes weight gain in mice
    Authors: F Talbot, CH Feetham, J Mokrosi?sk, K Lawler, JM Keogh, E Henning, E Mendes de, V Ayinampudi, S Saeed, A Bonnefond, M Arslan, GSH Yeo, P Froguel, DA Bechtold, A Adamson, N Humphreys, I Barroso, SM Luckman, IS Farooqi
    Nature Communications, 2023-03-15;14(1):1450.  2023-03-15
  7. Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells
    Authors: ML Hart, E Quon, ABG Vigil, IA Engstrom, OJ Newsom, K Davidsen, P Hoellerbau, SM Carlisle, LB Sullivan
    Elife, 2023-03-08;12(0):.  2023-03-08
  8. Structure-guided approach to modulate small molecule binding to a promiscuous ligand-activated protein
    Authors: W Lin, AD Huber, S Poudel, Y Li, J Seetharama, DJ Miller, T Chen
    Proceedings of the National Academy of Sciences of the United States of America, 2023-02-27;120(10):e2217804120.  2023-02-27
  9. Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells
    Authors: C Wang, R Nistala, M Cao, Y Pan, M Behrens, D Doll, RD Hammer, P Nistala, HM Chang, ETH Yeh, X Kang
    Cell Reports, 2023-02-17;42(2):112105.  2023-02-17
  10. Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation
    Authors: M Jia, Y Wang, J Wang, D Qin, M Wang, L Chai, Y Fu, C Zhao, C Gao, J Jia, W Zhao
    Nature Communications, 2023-02-07;14(1):660.  2023-02-07
  11. Polypharmacology-based kinome screen identifies new regulators of KSHV reactivation
    Authors: AT Olson, Y Kang, AM Ladha, CB Lim, M Lagunoff, TS Gujral, AP Geballe
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.  2023-02-01
  12. Combined anti-C1-INH and radiotherapy against glioblastoma
    Authors: E Liljedahl, E Konradsson, E Gustafsson, KF Jonsson, JK Olofsson, K Osther, C Ceberg, HN Redebrandt
    BMC Cancer, 2023-01-30;23(1):106.  2023-01-30
  13. CDK1 bridges NF-kappaB and beta-catenin signaling in response to H.�pylori infection in gastric tumorigenesis
    Authors: S Zhu, M Al-Mathkou, L Cao, S Khalafi, Z Chen, J Poveda, D Peng, H Lu, M Soutto, T Hu, OG McDonald, A Zaika, W El-Rifai
    Cell Reports, 2023-01-21;42(1):112005.  2023-01-21
  14. Nicotinamide riboside kinase 1 protects against diet and age-induced pancreatic beta-cell failure
    Authors: A Cercillieu, J Ratajczak, M Joffraud, JL Sanchez-Ga, G Jacot, A Zollinger, S Métairon, J Giroud-Ger, M Rumpler, E Ciarlo, M Valera-Alb, A Sambeat, C Canto
    Molecular Metabolism, 2022-09-20;0(0):101605.  2022-09-20
  15. Long-term anti-tumor effects following both conventional radiotherapy and FLASH in fully immunocompetent animals with glioblastoma
    Authors: E Liljedahl, E Konradsson, E Gustafsson, KF Jonsson, JK Olofsson, C Ceberg, HN Redebrandt
    Scientific Reports, 2022-07-19;12(1):12285.  2022-07-19
  16. In vitro radiosensitization of breast cancer with hypoxia-activated prodrugs
    Authors: R Aiyappa-Ma, L Elsalem, AIM Ibrahim, K Pors, SG Martin
    Journal of Cellular and Molecular Medicine, 2022-07-16;0(0):.  2022-07-16
  17. Cell-autonomous Hedgehog signaling controls Th17 polarization and pathogenicity
    Authors: J Hanna, F Beke, LM O'Brien, C Kapeni, HC Chen, V Carbonaro, AB Kim, K Kishore, TE Adolph, MO Skjoedt, K Skjoedt, M de la Roch, M de la Roch
    Nature Communications, 2022-07-14;13(1):4075.  2022-07-14
  18. Comparable Long-Term Tumor Control for Hypofractionated FLASH Versus Conventional Radiation Therapy in an Immunocompetent Rat Glioma Model
    Authors: E Konradsson, E Liljedahl, E Gustafsson, G Adrian, S Beyer, SE Ilaahi, K Petersson, C Ceberg, H Nittby Red
    Advances in radiation oncology, 2022-07-02;7(6):101011.  2022-07-02
  19. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022-05-13;0(0):102029.  2022-05-13
  20. Metformin combined with local irradiation provokes abscopal effects in a murine rectal cancer model
    Authors: M Tojo, H Miyato, K Koinuma, H Horie, H Tsukui, Y Kimura, Y Kaneko, H Ohzawa, H Yamaguchi, K Yoshimura, AK Lefor, N Sata, J Kitayama
    Scientific Reports, 2022-05-04;12(1):7290.  2022-05-04
  21. Molecular basis of crosstalk in nuclear receptors: heterodimerization between PXR and CAR and the implication in gene regulation
    Authors: MN Bwayi, E Garcia-Mal, SC Chai, B Xie, S Chodankar, AD Huber, J Wu, K Annu, WC Wright, HM Lee, J Seetharama, J Wang, CD Buchman, J Peng, T Chen
    Nucleic Acids Research, 2022-04-08;0(0):.  2022-04-08
  22. [89Zr]ZrDFO-CR011 PET Correlates with Response to Glycoprotein Nonmetastatic Melanoma B-targeted Therapy in Triple-negative Breast Cancer
    Authors: S Lee, A Cavaliere, JD Gallezot, T Keler, SK Michelhaug, E Belitzky, M Liu, T Mulnix, SE Maher, ALM Bothwell, F Li, M Phadke, S Mittal, B Marquez-No
    Molecular Cancer Therapeutics, 2022-03-01;21(3):440-447.  2022-03-01
  23. Galvanic current activates the NLRP3 inflammasome to promote Type I collagen production in tendon
    Authors: A Peñin-Fran, JA García-Vid, CM Martínez, P Escolar-Re, RM Martínez-O, AI Gómez, JM Bueno, F Minaya-Muñ, F Valera-Gar, F Medina-Mir, P Pelegrín
    Elife, 2022-02-24;11(0):.  2022-02-24
  24. Combining p53 mRNA nanotherapy with immune checkpoint blockade reprograms the immune microenvironment for effective cancer therapy
    Authors: Y Xiao, J Chen, H Zhou, X Zeng, Z Ruan, Z Pu, X Jiang, A Matsui, L Zhu, Z Amoozgar, DS Chen, X Han, DG Duda, J Shi
    Nature Communications, 2022-02-09;13(1):758.  2022-02-09
  25. Evolutionary analyses of the gasdermin family suggest conserved roles in infection response despite loss of pore-forming functionality
    Authors: D Angosto-Ba, C Alarcón-Vi, L Hurtado-Na, MC Baños, J Rivers-Aut, P Pelegrín
    Bmc Biology, 2022-01-07;20(1):9.  2022-01-07
  26. Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2
    Authors: Y Gu, J Cao, X Zhang, H Gao, Y Wang, J Wang, J He, X Jiang, J Zhang, G Shen, J Yang, X Zheng, G Hu, Y Zhu, S Du, Y Zhu, R Zhang, J Xu, F Lan, D Qu, G Xu, Y Zhao, D Gao, Y Xie, M Luo, Z Lu
    Cell Research, 2021-11-26;0(0):.  2021-11-26
  27. A novel strategy for combination of clofarabine and pictilisib is synergistic in gastric cancer
    Authors: S Khalafi, S Zhu, R Khurana, I Lohse, S Giordano, S Corso, H Al-Ali, SP Brothers, C Wahlestedt, S Schürer, W El-Rifai
    Translational Oncology, 2021-11-01;15(1):101260.  2021-11-01
  28. Autophagy-competent mitochondrial translation elongation factor TUFM inhibits caspase-8-mediated apoptosis
    Authors: CY Choi, MT Vo, J Nicholas, YB Choi
    Cell Death and Differentiation, 2021-09-12;0(0):.  2021-09-12
  29. A Novel Monoclonal Antibody Targeting Cancer-Specific Plectin Has Potent Antitumor Activity in Ovarian Cancer
    Authors: SM Perez, J Dimastroma, CN Landen, KA Kelly
    Cells, 2021-08-27;10(9):.  2021-08-27
  30. Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancers
    Authors: N Mao, Z Zhang, YS Lee, D Choi, AA Rivera, D Li, C Lee, S Haywood, X Chen, Q Chang, G Xu, HA Chen, E de Stanchi, C Sawyers, N Rosen, AC Hsieh, Y Chen, BS Carver
    Nature Communications, 2021-08-20;12(1):5053.  2021-08-20
  31. Mutant APC promotes tumor immune evasion via PD-L1 in colorectal cancer
    Authors: B Cen, J Wei, D Wang, Y Xiong, JW Shay, RN DuBois
    Oncogene, 2021-08-12;0(0):.  2021-08-12
  32. Tumor-conditional IL-15 pro-cytokine reactivates anti-tumor immunity with limited toxicity
    Authors: J Guo, Y Liang, D Xue, J Shen, Y Cai, J Zhu, YX Fu, H Peng
    Cell Research, 2021-08-10;0(0):.  2021-08-10
  33. Exosomal miR-1260b derived from non-small cell lung cancer promotes tumor metastasis through the inhibition of HIPK2
    Authors: DH Kim, H Park, YJ Choi, MH Kang, TK Kim, CG Pack, CM Choi, JC Lee, JK Rho
    Cell Death & Disease, 2021-07-28;12(8):747.  2021-07-28
  34. Long-Chain Acylcarnitines Decrease the Phosphorylation of the Insulin Receptor at Tyr1151 Through a PTP1B-Dependent Mechanism
    Authors: K Vilks, M Videja, M Makrecka-K, M Katkevics, E Sevostjano, A Grandane, M Dambrova, E Liepinsh
    International Journal of Molecular Sciences, 2021-06-16;22(12):.  2021-06-16
  35. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer
    Authors: Z Wang, Y Goto, MM Allevato, VH Wu, R Saddawi-Ko, M Gilardi, D Alvarado, BS Yung, A O'Farrell, AA Molinolo, U Duvvuri, JR Grandis, JA Califano, EEW Cohen, JS Gutkind
    Nature Communications, 2021-04-22;12(1):2383.  2021-04-22
  36. Liquid biopsy genotyping by a simple lateral flow strip assay with visual detection
    Authors: P Kalligosfy, S Nikou, V Bravou, DP Kalogianni
    Analytica chimica acta, 2021-04-12;1163(0):338470.  2021-04-12
  37. The Angiotensin II Receptor Blocker Losartan Sensitizes Human Liver Cancer Cells to Lenvatinib-Mediated Cytostatic and Angiostatic Effects
    Authors: H Takagi, K Kaji, N Nishimura, K Ishida, H Ogawa, H Takaya, H Kawaratani, K Moriya, T Namisaki, T Akahane, A Mitoro, H Yoshiji
    Cells, 2021-03-05;10(3):.  2021-03-05
  38. Mesenchymal stem cell-derived interleukin-28 drives the selection of apoptosis resistant bone metastatic prostate cancer
    Authors: JJ McGuire, JS Frieling, CH Lo, T Li, A Muhammad, HR Lawrence, NJ Lawrence, LM Cook, CC Lynch
    Nature Communications, 2021-02-01;12(1):723.  2021-02-01
  39. NAD+ boosting reduces age-associated amyloidosis and restores mitochondrial homeostasis in muscle
    Authors: M Romani, V Sorrentino, CM Oh, H Li, TI de Lima, H Zhang, M Shong, J Auwerx
    Cell Reports, 2021-01-19;34(3):108660.  2021-01-19
  40. MAOA promotes prostate cancer cell perineural invasion through SEMA3C/PlexinA2/NRP1-cMET signaling
    Authors: L Yin, J Li, J Wang, T Pu, J Wei, Q Li, BJ Wu
    Oncogene, 2021-01-08;0(0):.  2021-01-08
  41. Intravital molecular imaging reveals the restrained capacity of CTLs in the killing of tumor cells in the liver
    Authors: L Liu, B Dai, R Li, Z Liu, Z Zhang
    Theranostics, 2021-01-01;11(1):194-208.  2021-01-01
  42. The ITIM-Containing Receptor: Leukocyte-Associated Immunoglobulin-Like Receptor-1 (LAIR-1) Modulates Immune Response and Confers Poor Prognosis in Invasive Breast Carcinoma
    Authors: C Joseph, MA Alsaleem, MS Toss, YA Kariri, M Althobiti, S Alsaeed, AI Aljohani, PL Narasimha, NP Mongan, AR Green, EA Rakha
    Cancers, 2020-12-30;13(1):.  2020-12-30
  43. Evaluation of chemotherapy and P2Et extract combination in ex-vivo derived tumor mammospheres from breast cancer patients
    Authors: C Urueña, TA Sandoval, P Lasso, M Tawil, A Barreto, L Torregrosa, S Fiorentino
    Sci Rep, 2020-11-12;10(1):19639.  2020-11-12
  44. Epigenetic targeting of neuropilin-1 prevents bypass signaling in drug-resistant breast cancer
    Authors: A Abdullah, SS Akhand, JSP Paez, W Brown, L Pan, S Libring, M Badamy, E Dykuizen, L Solorio, W Andy Tao, MK Wendt
    Oncogene, 2020-10-30;0(0):.  2020-10-30
  45. Cooling-mediated protection from chemotherapy drug-induced cytotoxicity in human keratinocytes by inhibition of cellular drug uptake
    Authors: C Dunnill, K Ibraheem, M Peake, M Ioannou, M Palmer, A Smith, A Collett, NT Georgopoul
    PLoS ONE, 2020-10-15;15(10):e0240454.  2020-10-15
  46. Maintenance Therapy for ATM-Deficient Pancreatic Cancer by Multiple DNA Damage Response Interferences after Platinum-Based Chemotherapy
    Authors: E Roger, J Gout, F Arnold, AK Beutel, M Müller, A Abaei, TFE Barth, V Rasche, T Seufferlei, L Perkhofer, A Kleger
    Cells, 2020-09-16;9(9):.  2020-09-16
  47. BET, SRC, and BCL2 family inhibitors are synergistic drug combinations with PARP inhibitors in ovarian cancer
    Authors: GYL Lui, R Shaw, FX Schaub, IN Stork, KE Gurley, C Bridgwater, RL Diaz, R Rosati, HA Swan, TA Ince, TC Harding, VK Gadi, BA Goff, CJ Kemp, EM Swisher, C Grandori
    EBioMedicine, 2020-09-11;60(0):102988.  2020-09-11
  48. Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies
    Authors: A Anloague, A Mahoney, O Ogunbekun, TA Hiland, WR Thompson, B Larsen, MT Loghmani, JM Hum, JW Lowery
    BMC Res Notes, 2020-08-27;13(1):400.  2020-08-27
  49. Novel oral plasminogen activator inhibitor?1 inhibitor TM5275 attenuates hepatic fibrosis under metabolic syndrome via suppression of activated hepatic stellate cells in rats
    Authors: R Noguchi, K Kaji, T Namisaki, K Moriya, H Kawaratani, M Kitade, H Takaya, Y Aihara, A Douhara, K Asada, N Nishimura, T Miyata, H Yoshiji
    Molecular Medicine Reports, 2020-07-28;0(0):.  2020-07-28
  50. Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage
    Authors: X Fan, Z Hao, Z Li, X Wang, J Wang
    Mediators Inflamm., 2020-07-21;2020(0):6126490.  2020-07-21
  51. Comprehensive RNA-Seq profiling of the lung transcriptome of Bashbay sheep in response to experimental Mycoplasma ovipneumoniae infection
    Authors: Z Du, Y Sun, J Wang, H Liu, Y Yang, N Zhao
    PLoS ONE, 2020-07-08;15(7):e0214497.  2020-07-08
  52. Redox homeostasis maintained by GPX4 facilitates STING activation
    Authors: M Jia, D Qin, C Zhao, L Chai, Z Yu, W Wang, L Tong, L Lv, Y Wang, J Rehwinkel, J Yu, W Zhao
    Nat. Immunol., 2020-06-15;21(7):727-735.  2020-06-15
  53. Loss of the transcription factor MAFB limits beta-cell derivation from human PSCs
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  54. Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity
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    PLoS Pathog., 2020-05-15;16(5):e1008572.  2020-05-15
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FAQs

  1. What are recommendations for cell culture numbers prior to evaluating supernatant in the kit?

    • For adherent cells, we recommend culturing cells to confluence prior to the assay. For suspension cells, we recommend culturing to a density of 0.5-1 x 106 cells/mL.

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Reviews for MycoProbe Mycoplasma Detection Kit

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MycoProbe Mycoplasma Detection Kit
By Vincent Giacalone on 05/20/2021

This is a alternative to PCR for mycoplasma testing. I used it to test cell culture supernatants but can also be used for cell lysates. The positive control wells are in red and negative control are in green. 14 samples were measured in duplicate and after subtracting the negative control all were well below the lower threshold of 0.05. The kit assay requires about 6 hours to complete but mostly consists of incubation steps, one of which requires 65 degree water bath. The one downside is the kit is best for measuring large batches of samples all at once given the plate based format.


MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/05/2020

This is the best kit I have ever used for testing mycoplasma, better than PCR and fluorescence based assays. It can test both cells and media, both frozen and fresh samples. It does not require 2 weeks antibiotic free culture time. It takes about 5 to 6 hours to finish. The only not very convenient step is the 65C incubation in water bath.


MycoProbe Mycoplasma Detection Kit
By Anonymous on 12/06/2019

MycoProbe Mycoplasma Detection Kit
By Leslie Priddy on 04/03/2018

MycoProbe Mycoplasma Detection Kit
By Anonymous on 07/07/2017

MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/13/2016

Our lab has been using this kit for routine Mycoplasma testing of cultures for over 2 years now. The kit is highly sensitive and specific to Mycoplasma. The data is reproducible and more accurate than other tests, the size of the assay is adjustable (the kit comes as 12 8-well strips). You get reliable results in a few hours. Overall, great product and highly recommended.