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MycoProbe Mycoplasma Detection Kit

CUL001B 74 Citations 6 Reviews Datasheet / COA / SDS
Mycoplasmas Detected: M. hyorhinis, M. arginini, M. fermentans, M. orale, M. pirum, M. hominis, M. salivarium, Acholeplasma laidlawii
Catalog # Availability Size / Price Qty
CUL001B
Mycoplasma Detection in Cell Line Supernates and Cell Lysates. 
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Product Details
Procedure
Citations (74)
FAQs
Reviews (6)
Summary Product Datasheets Data Images

MycoProbe Mycoplasma Detection Kit Summary

Kit Summary

A complete kit to detect common antibiotic-resistant cell culture contaminants

Key Benefits

  • Reduces experimental variation
  • Accurate and highly sensitive
  • Simple multiwell-based colorimetric assay
  • Only takes 4.5 hours


View Data Examples

 

 

Why should I be worried about mycoplasma contamination?

Mycoplasma contamination affects up to 80% of continuous cell cultures. If undetected, mycoplasma contamination can have significant effects on the quality and reliability of your cell culture preparations.

Mycoplasma contamination:

  • Induces deleterious effects on cell culture quality.
  • Alters the phenotypic characteristics of host cells.
  • Increases experimental variation.
  • Is common in eukaryotic cell cultures.
  • Is resistant to antibiotics such as penicillin and streptomycin.
  • Has multiple sources (i.e. personnel, reagents, other infected cells).

Mycoplasma are difficult to detect because they:

  • Are not visible using standard microscopes.
  • Are small enough to pass through 0.45 mm sterilization filters.
  • Do not produce changes in culture medium color, pH, or turbidit

What is the best method to determine if my cultured cells are mycoplasma-contaminated?

To provide an accurate and highly sensitive tool for routine screening of mycoplasma contamination in cultured cells, R&D Systems developed the MycoProbe Mycoplasma Detection Assay. This assay detects Mycoplasma 16S ribosomal RNA (rRNA) using a colorimetric signal amplification system with sensitivity comparable to PCR. The MycoProbe assay is not susceptible to common problems encountered with PCR-based mycoplasma detection kits.

The MycoProbe Mycoplasma Detection Assay:

  • Detects the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination.
  • Is highly sensitive (comparable to PCR).
  • Is compatible with high-throughput screening.
  • Does not generate false positives from amplicon contamination.
  • Can be used for cell culture supernatants or cultured cell pellets.
  • Can be used with samples from fresh or frozen cells.
  • Does not require cells to be cultured in antibiotic-free media.
  • Includes a synthetic DNA oligonucleotide positive control.
  • Generates results in 4.5 hours.

Alternative Methods for Mycoplasma Detection

Technique

Advantage

Disadvantage
Microbiological culture Most sensitive
  • Takes 2 to 4 weeks
  • Cannot detect fastidious mycoplasma
  • Requires specialized laboratory conditions
Fluorescent DNA staining Efficient
  • Cannot detect mycoplasma that cyto-absorb poorly
ELISA of cell surface antigens Common technique
  • Low sensitivity
PCR-based detection High sensitivity
  • Prone to both false positives and false negatives
Biochemical activity Common technique
  • Inconsistent results across cell lines
Kit Components

The MycoProbeTM Mycoplasma Detection Kit (Catalog # CUL001B) contains enough reagents to assay one 96-well plate for mycoplasma contamination.

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips
Precautions

The Wash Buffer supplied in this kit contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

When handling cell culture samples, appropriate precautions should be taken to prevent exposure to mycoplasma and other hazardous biological agents.

Data Examples

View Larger Image

Mycoplasma Detection in Cell Line Supernates and Cell Lysates. The presence of the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination was tested in supernates and cell lysates of the indicated cell lines using the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B). The average of the duplicate optical density (OD) readings for each control and sample was determined. The average negative control OD value was subtracted from all average sample OD values. A calculated positive control OD value of >0.10 indicated mycoplasma contamination (black line) in the CTLL-2, BaF3, A431, and K562 (cell lysate 1) samples. Abbreviations: CTLL-2 mouse cytotoxic T cell line, BaF3 mouse pro-B cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

Product Datasheet
COA
SDS

Data Example

Mycoplasma Detection Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  View Larger

Mycoplasma Detection in Cell Line Supernates and Cell Lysates.  The presence of the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination was tested in supernates and cell lysates of the indicated cell lines using the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B). The average of the duplicate optical density (OD) readings for each control and sample was determined. The average negative control OD value was subtracted from all average sample OD values. A calculated positive control OD value of >0.10 indicated mycoplasma contamination (black line) in the CTLL-2, BaF3, A431, and K562 (cell lysate 1) samples. Abbreviations: CTLL-2 mouse cytotoxic T cell line, BaF3 mouse pro-B cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mycoplasma contamination can be evaluated in cell culture supernates or cell pellets using this straightforward procedure:

  • Samples are lysed and hybridized with biotin-labeled capture oligonucleotide probes
  • Digoxigenin-labeled detection probes target the eight most common mycoplasma contaminants
  • Following signal amplification, multiwells are measured using a standard colorimetric plate reader
  • Results are generated in 4.5 hours
CUL001B Protocol Graphic Summary
View Graphic Procedure
 

 

Reagents Provided

Reagents supplied in the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B):

  • Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution
  • Hybridization Plate - One 96 well polystyrene microplate
  • Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin
  • Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives
  • Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives
  • Capture Probes - 1.1 mL of a six-fold concentrated stock solution
  • Detection Probes - 1.1 mL of a six-fold concentrated stock solution
  • Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide
  • Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives
  • Substrate - 1 vial of lyophilized NADPH with stabilizers
  • Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers
  • Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers
  • Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers
  • Stop Solution - 6 mL of 2 N sulfuric acid
  • Float Collar - Microplate float collar for water bath
  • Plate Sealers - 12 adhesive strips

 

Other Supplies Required

Reagents

  • Deionized water, RNase-free
  • Cell samples of interest

Materials

  • Pipettes and pipette tips
  • Squirt bottle or manifold dispenser
  • 100 mL and 1,000 mL graduated cylinders for preparation of Wash Buffer
  • Gloves and mask

Equipment

  • Microplate reader capable of measuring absorbance at 490 nm with the correction wavelength set at 650 nm or 690 nm
  • Horizontal orbital microplate shaker (0.12" orbit) capable of maintaining a speed of 500 + 50 rpm
  • 65 + 1 °C water bath
  • Vortex mixer

 

Procedure Overview

R&D Systems Protocol for Mouse Treg Cell Differentiation

Wash the Hybridization Plate 2 times with Wash Buffer.

Add diluted Probes, Positive Control, Sample Diluent (Negative Control), or sample to the designated wells.

Incubate the plate for 60 minutes in a 65 °C water bath.

Protocol for Mouse Treg Cell Differentiation Step 1

Wash the Streptavidin Plate 2 times with Wash Buffer.

Transfer 150 µL from each well of the Hybridization Plate to the Streptavidin Plate.

Incubate for 60 minutes on a horizontal orbital shaker.

Protocol for Mouse Treg Cell Differentiation Step 2

Wash the Streptavidin Plate 4 times with Wash Buffer.

Add Anti-Digoxigenin Conjugate to each well.

Incubate for 60 minutes on a shaker.

Protocol for Mouse Treg Cell Differentiation Step 3

Wash the Streptavidin Plate 6 times with Wash Buffer.

Add Substrate Solution to each well.

Incubate for 60 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 4

Add Amplifier Solution to each well.

Incubate for 30 minutes on a shaker.
Do not wash.

Protocol for Mouse Treg Cell Differentiation Step 5

Add Stop Solution to each well.

Determine the optical density (OD) of each well within 30 minutes, using a microplate reader set to 490 nm.

Protocol for Mouse Treg Cell Differentiation Step 6

Note: If wavelength correction is available, set to 650 nm or 690 nm. If wavelength correction is not available, subtract readings at 650 nm or 690 nm from the readings at 490 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 490 nm without correction may be higher and less accurate.

 
 
Calculation of Results

Determine the average of the duplicate optical density (OD) readings for each control and sample. Subtract the average negative control OD value from all average OD values. The calculated positive control OD value should be > 1.5.

OD Values (Calculated)

Result

Interpretation
< 0.05 Negative No mycoplasma detected
0.05 – 0.10 Inconclusive Sample is suspect for mycoplasma. Continue to culture for an additional 2 - 3 days and repeat the test. If sample gives a similar OD, then no mycoplasma are detected.
> 0.10 Positive Mycoplasma detected

Citations for MycoProbe Mycoplasma Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

74 Citations: Showing 1 - 10
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  1. Novel oral plasminogen activator inhibitor?1 inhibitor TM5275 attenuates hepatic fibrosis under metabolic syndrome via suppression of activated hepatic stellate cells in rats
    Authors: R Noguchi, K Kaji, T Namisaki, K Moriya, H Kawaratani, M Kitade, H Takaya, Y Aihara, A Douhara, K Asada, N Nishimura, T Miyata, H Yoshiji
    Molecular Medicine Reports, 2020;0(0):.  2020
  2. Comprehensive RNA-Seq profiling of the lung transcriptome of Bashbay sheep in response to experimental Mycoplasma ovipneumoniae infection
    Authors: Z Du, Y Sun, J Wang, H Liu, Y Yang, N Zhao
    PLoS ONE, 2020;15(7):e0214497.  2020
  3. CD73 blockade enhances the local and abscopal effects of radiotherapy in a murine rectal cancer model
    Authors: H Tsukui, H Horie, K Koinuma, H Ohzawa, Y Sakuma, Y Hosoya, H Yamaguchi, K Yoshimura, AK Lefor, N Sata, J Kitayama
    BMC Cancer, 2020;20(1):411.  2020
  4. Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity
    Authors: HL Mercer, LM Snyder, CM Doherty, BA Fox, DJ Bzik, EY Denkers
    PLoS Pathog., 2020;16(5):e1008572.  2020
  5. MAFB promotes cancer stemness and tumorigenesis in osteosarcoma through a Sox9-mediated positive feedback loop
    Authors: Y Chen, B Wang, M Huang, T Wang, C Hu, Q Liu, D Han, C Chen, J Zhang, Z Li, C Liu, W Lei, Y Chang, M Wu, D Xiang, Y Chen, R Wang, W Huang, Z Lei, X Chu
    Cancer Res., 2020;0(0):.  2020
  6. EIF3H orchestrates Hippo pathway-mediated oncogenesis via catalytic control of YAP stability
    Authors: Z Zhou, H Zhou, L Ponzoni, A Luo, R Zhu, M He, Y Huang, KL Guan, I Bahar, Z Liu, Y Wan
    Cancer Res., 2020;0(0):.  2020
  7. KANSL2 and MBNL3 are regulators of pancreatic ductal adenocarcinoma invasion
    Authors: PO Oladimeji, J Bakke, WC Wright, T Chen
    Sci Rep, 2020;10(1):1485.  2020
  8. &alpha-Linolenic acid but not linolenic acid protects against hypertension: critical role of SIRT3 and autophagic flux
    Authors: G Li, X Wang, H Yang, P Zhang, F Wu, Y Li, Y Zhou, X Zhang, H Ma, W Zhang, J Li
    Cell Death Dis, 2020;11(2):83.  2020
  9. Oncogenic ERG represses PI3K signaling through down-regulation of IRS2
    Authors: N Mao, D Gao, W Hu, S Gadal, H Hieronymus, S Wang, YS Lee, P Sullivan, Z Zhang, D Choi, N Rosen, CL Sawyers, A Gopalan, Y Chen, BS Carver
    Cancer Res., 2020;0(0):.  2020
  10. Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage
    Authors: X Fan, Z Hao, Z Li, X Wang, J Wang
    Mediators Inflamm., 2020;2020(0):6126490.  2020
  11. Maintenance Therapy for ATM-Deficient Pancreatic Cancer by Multiple DNA Damage Response Interferences after Platinum-Based Chemotherapy
    Authors: E Roger, J Gout, F Arnold, AK Beutel, M Müller, A Abaei, TFE Barth, V Rasche, T Seufferlei, L Perkhofer, A Kleger
    Cells, 2020;9(9):.  2020
  12. Epigenetic targeting of neuropilin-1 prevents bypass signaling in drug-resistant breast cancer
    Authors: A Abdullah, SS Akhand, JSP Paez, W Brown, L Pan, S Libring, M Badamy, E Dykuizen, L Solorio, W Andy Tao, MK Wendt
    Oncogene, 2020;0(0):.  2020
  13. Cooling-mediated protection from chemotherapy drug-induced cytotoxicity in human keratinocytes by inhibition of cellular drug uptake
    Authors: C Dunnill, K Ibraheem, M Peake, M Ioannou, M Palmer, A Smith, A Collett, NT Georgopoul
    PLoS ONE, 2020;15(10):e0240454.  2020
  14. Aberrant expression of ERG promotes resistance to combined PI3K and AR pathway inhibition through maintenance of AR target genes
    Authors: N Mao, D Gao, W Hu, H Hieronymus, S Wang, YS Lee, C Lee, D Choi, A Gopalan, Y Chen, BS Carver
    Mol. Cancer Ther., 2019;0(0):.  2019
  15. CD38-driven mitochondrial trafficking promotes bioenergetic plasticity in multiple myeloma
    Authors: CR Marlein, RE Piddock, JJ Mistry, L Zaitseva, C Hellmich, RH Horton, Z Zhou, MJ Auger, KM Bowles, SA Rushworth
    Cancer Res., 2019;0(0):.  2019
  16. Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells
    Authors: J Bakke, WC Wright, AE Zamora, P Oladimeji, JC Crawford, CT Brewer, RJ Autry, WE Evans, PG Thomas, T Chen
    BMC Cancer, 2019;19(1):253.  2019
  17. Activation of NIX-mediated mitophagy by an interferon regulatory factor homologue of human herpesvirus
    Authors: MT Vo, BJ Smith, J Nicholas, YB Choi
    Nat Commun, 2019;10(1):3203.  2019
  18. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson's Disease Model
    Authors: J Han, SJ Kim, MJ Ryu, Y Jang, MJ Lee, X Ju, YL Lee, J Cui, M Shong, JY Heo, GR Kweon
    Oxid Med Cell Longev, 2019;2019(0):4174803.  2019
  19. Influence of Vitamin D on Corneal Epithelial Cell Desmosomes and Hemidesmosomes
    Authors: X Lu, MA Watsky
    Invest. Ophthalmol. Vis. Sci., 2019;60(13):4074-4083.  2019
  20. Genome-wide miRNA profiling and pivotal roles of miRs 125a-5p and 17-92 cluster in human neutrophil maturation and differentiation of acute myeloid leukemia cells
    Authors: EH Dakir, F Mollinedo
    Oncotarget, 2019;10(51):5313-5331.  2019
  21. Centrosome amplification in cancer disrupts autophagy and sensitizes to autophagy inhibition
    Authors: RA Denu, G Kaur, MM Sass, A Lakkaraju, ME Burkard
    Mol. Cancer Res., 2019;0(0):.  2019
  22. Delivery of mRNA vaccines with heterocyclic lipids increases anti-tumor efficacy by STING-mediated immune cell activation
    Authors: L Miao, L Li, Y Huang, D Delcassian, J Chahal, J Han, Y Shi, K Sadtler, W Gao, J Lin, JC Doloff, R Langer, DG Anderson
    Nat. Biotechnol., 2019;0(0):.  2019
  23. Smooth muscle glucose metabolism promotes monocyte recruitment and atherosclerosis in a mouse model of metabolic syndrome
    Authors: VZ Wall, S Barnhart, JE Kanter, F Kramer, M Shimizu-Al, N Adhikari, TN Wight, JL Hall, KE Bornfeldt
    JCI Insight, 2018;3(11):.  2018
  24. A combination of SAHA and Quinacrine is effective in inducing cancer cell death in upper gastrointestinal cancers
    Authors: S Zhu, Z Chen, L Wang, D Peng, A Belkhiri, AC Lockhart, W El-Rifai
    Clin. Cancer Res., 2018;0(0):.  2018
  25. A Novel Strategy to Prevent Advanced Atherosclerosis and Lower Blood Glucose in a Mouse Model of Metabolic Syndrome
    Authors: JE Kanter, F Kramer, S Barnhart, JM Duggan, M Shimizu-Al, V Kothari, A Chait, SD Bouman, JA Hamerman, BF Hansen, GS Olsen, KE Bornfeldt
    Diabetes, 2018;0(0):.  2018
  26. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018;7(0):.  2018
  27. The anti-psychotic drug pimozide is a novel chemotherapeutic for breast cancer
    Authors: EH Dakir, A Pickard, K Srivastava, CM McCrudden, SR Gross, S Lloyd, SD Zhang, A Margariti, R Morgan, PS Rudland, M El-Tanani
    Oncotarget, 2018;9(79):34889-34910.  2018
  28. miR-663a inhibits tumor growth and invasion by regulating TGF-?1 in hepatocellular carcinoma
    Authors: C Zhang, B Chen, A Jiao, F Li, N Sun, G Zhang, J Zhang
    BMC Cancer, 2018;18(1):1179.  2018
  29. Targeting PARP1 in XRCC1 deficient sporadic invasive breast cancer or pre-invasive ductal carcinoma in situ induces synthetic lethality and chemoprevention
    Authors: R Ali, A Al-Kawaz, MS Toss, AR Green, IM Miligy, KA Mesquita, C Seedhouse, S Mirza, V Band, EA Rakha, S Madhusudan
    Cancer Res., 2018;0(0):.  2018
  30. De novo NAD+ synthesis enhances mitochondrial function and improves health
    Authors: E Katsyuba, A Mottis, M Zietak, F De Franco, V van der Ve, K Gariani, D Ryu, L Cialabrini, O Matilainen, P Liscio, N Giacchè, N Stokar-Reg, D Legouis, S de Seigneu, J Ivanisevic, N Raffaelli, K Schoonjans, R Pellicciar, J Auwerx
    Nature, 2018;0(0):.  2018
  31. Lineage dynamics of murine pancreatic development at single-cell resolution
    Authors: LE Byrnes, DM Wong, M Subramania, NP Meyer, CL Gilchrist, SM Knox, AD Tward, CJ Ye, JB Sneddon
    Nat Commun, 2018;9(1):3922.  2018
  32. Raman micro-spectroscopy for accurate identification of primary human bronchial epithelial cells
    Authors: JM Surmacki, BJ Woodhams, A Haslehurst, BAJ Ponder, SE Bohndiek
    Sci Rep, 2018;8(1):12604.  2018
  33. Complex bile duct network formation within liver decellularized extracellular matrix hydrogels
    Authors: PL Lewis, J Su, M Yan, F Meng, SS Glaser, GD Alpini, RM Green, B Sosa-Pined, RN Shah
    Sci Rep, 2018;8(1):12220.  2018
  34. TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue
    Authors: LA Velazquez-, A Perino, V Lemos, M Zietak, M Nomura, TWH Pols, K Schoonjans
    Nat Commun, 2018;9(1):245.  2018
  35. The ATR inhibitor AZD6738 synergizes with gemcitabine in vitro and in vivo to induce pancreatic ductal adenocarcinoma regression
    Authors: Y Wallez, CR Dunlop, TI Johnson, SB Koh, C Fornari, JW Yates, S Bernaldo d, A Lau, FM Richards, DI Jodrell
    Mol. Cancer Ther., 2018;0(0):.  2018
  36. Integration of distinct ShcA signaling complexes promotes breast tumor growth and tyrosine kinase inhibitor resistance
    Authors: JR Ha, R Ahn, HW Smith, V Sabourin, S H?bert, E Cepeda Cañ, YK Im, C Kleinman, WJ Muller, J Ursini-Sie
    Mol. Cancer Res., 2018;0(0):.  2018
  37. Screening, large-scale production and structure-based classification of cystine-dense peptides
    Authors: CE Correnti, MM Gewe, C Mehlin, AD Bandaranay, WA Johnsen, PB Rupert, MY Brusniak, M Clarke, SE Burke, W De Van Der, K Pilat, SM Turnbaugh, D May, A Watson, MK Chan, CD Bahl, JM Olson, RK Strong
    Nat. Struct. Mol. Biol., 2018;25(3):270-278.  2018
  38. Effects of Exposure to Acetaminophen and Ibuprofen on Fetal Germ Cell Development in Both Sexes in Rodent and Human Using Multiple Experimental Systems
    Authors: P Hurtado-Go, RA Anderson, J Macdonald, S van den Dr, K Kilcoyne, A Jørgensen, C McKinnell, S Macpherson, RM Sharpe, RT Mitchell
    Environ. Health Perspect., 2018;126(4):047006.  2018
  39. HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome
    Authors: S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce
    Oncogene, 2018;0(0):.  2018
  40. Mechanistic distinctions between CHK1 and WEE1 inhibition guide the scheduling of triple therapy with gemcitabine
    Authors: SB Koh, Y Wallez, CR Dunlop, S Bernaldo d, TE Bapiro, FM Richards, DI Jodrell
    Cancer Res., 2018;0(0):.  2018
  41. O-GlcNAcylation of the tumor suppressor FOXO3 triggers aberrant cancer cell growth
    Authors: H Shin, HJ Cha, K Na, MJ Lee, JY Cho, CY Kim, EK Kim, CM Kang, H Kim, YK Paik
    Cancer Res., 2018;0(0):.  2018
  42. Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17
    Authors: JS Frieling, G Shay, V Izumi, ST Aherne, RG Saul, M Budzevich, J Koomen, CC Lynch
    Oncogene, 2017;0(0):.  2017
  43. CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition
    Authors: L Qiu, X Tan, J Lin, RY Liu, S Chen, R Geng, J Wu, W Huang
    J Cancer, 2017;8(13):2626-2635.  2017
  44. Lack of constitutively active DNA repair sensitizes glioblastomas to Akt inhibition and induces synthetic lethality with radiation treatment in a p53-dependentmanner
    Authors: K Palanicham, D Patel, JR Jacob, KT Litzenberg, N Gordon, K Acus, SE Noda, A Chakravart
    Mol. Cancer Ther., 2017;0(0):.  2017
  45. Transcription factor ZNF148 is a negative regulator of human muscle differentiation
    Authors: J Bakke, WC Wright, AE Zamora, SS Ong, YM Wang, JD Hoyer, CT Brewer, PG Thomas, T Chen
    Sci Rep, 2017;7(1):8138.  2017
  46. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.  2017
  47. Neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy
    Authors: GS Cho, DI Lee, E Tampakakis, S Murphy, P Andersen, H Uosaki, S Chelko, K Chakir, I Hong, K Seo, HV Chen, X Chen, C Basso, SR Houser, GF Tomaselli, B O'Rourke, DP Judge, DA Kass, C Kwon
    Cell Rep, 2017;18(2):571-582.  2017
  48. Glucose-dependent regulation of pregnane X receptor is modulated by AMP-activated protein kinase
    Authors: PO Oladimeji, W Lin, CT Brewer, T Chen
    Sci Rep, 2017;7(0):46751.  2017
  49. BRCA2 hypomorphic missense variants confer moderate risks of breast cancer
    Authors: H Shimelis, RL Mesman, C Von Nicola, A Ehlen, L Guidugli, C Martin, FM Calleja, H Meeks, E Hallberg, J Hinton, J Lilyquist, C Hu, CM Aalfs, K Aittomaki, IL Andrulis, H Anton-Culv, V Arndt, MW Beckmann, JJ Benitez, N Bogdanova, SE Bojesen, MK Bolla, AL Borresen-D, H Brauch, P Brennan, H Brenner, A Broeks, B Brouwers, T Bruning, B Burwinkel, J Chang-Clau, G Chenevix-T, CY Cheng, JY Choi, JM Collée, A Cox, SS Cross, K Czene, H Darabi, J Dennis, T Dork, I Dos Santos, AM Dunning, PA Fasching, JD Figueroa, H Flyger, M Garcia-Clo, GG Giles, G Glendon, P Guenel, CA Haiman, P Hall, U Hamann, M Hartman, FB Hogervorst, A Hollestell, JL Hopper, H Ito, A Jakubowska, D Kang, VM Kosma, V Kristensen, KN Lai, D Lambrechts, L Le Marchan, J Li, A Lindblom, A Lophatanan, J Lubinski, E Machackova, A Mannermaa, S Margolin, F Marme, K Matsuo, H Miao, K Michailido, RL Milne, K Muir, SL Neuhausen, H Nevanlinna, JE Olson, C Olswold, JC Oosterwijk, A Osorio, P Peterlongo, J Peto, PD Pharoah, K Pylkäs, P Radice, MU Rashid, V Rhenius, A Rudolph, S Sangrajran, EJ Sawyer, MK Schmidt, MJ Schoemaker, CM Seynaeve, M Shah, CY Shen, MJ Shrubsole, XO Shu, SL Slager, MC Southey, DO Stram, AJ Swerdlow, SH Teo, I Tomlinson, D Torres, T Truong, CJ van Aspere, LE van der Ko, Q Wang, R Winqvist, AH Wu, JC Yu, W Zheng, Y Zheng, J Leary, LC Walker, L Foretova, F Fostira, K Claes, L Varesco, S Moghadasi, DF Easton, AB Spurdle, P Devilee, H Vrieling, AN Monteiro, DE Goldgar, A Carreira, MP Vreeswijk, FJ Couch
    Cancer Res, 2017;0(0):.  2017
  50. HIF2? targeted RNAi therapeutic inhibits clear cell renal cell carcinoma
    Authors: SC Wong, W Cheng, H Hamilton, AL Nicholas, DH Wakefield, A Almeida, AV Blokhin, J Carlson, ZC Neal, V Subbotin, G Zhang, J Hegge, S Bertin, VS Trubetskoy, DB Rozema, DL Lewis, SB Kanner
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FAQs

  1. What are recommendations for cell culture numbers prior to evaluating supernatant in the kit?

    • For adherent cells, we recommend culturing cells to confluence prior to the assay. For suspension cells, we recommend culturing to a density of 0.5-1 x 106 cells/mL.

View all Cell Culture Product FAQs

Reviews for MycoProbe Mycoplasma Detection Kit

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MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/20/2021
Application:

This is a alternative to PCR for mycoplasma testing. I used it to test cell culture supernatants but can also be used for cell lysates. The positive control wells are in red and negative control are in green. 14 samples were measured in duplicate and after subtracting the negative control all were well below the lower threshold of 0.05. The kit assay requires about 6 hours to complete but mostly consists of incubation steps, one of which requires 65 degree water bath. The one downside is the kit is best for measuring large batches of samples all at once given the plate based format.

Serum MycoProbe Mycoplasma Detection Kit CUL001B
MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/05/2020
Application:

This is the best kit I have ever used for testing mycoplasma, better than PCR and fluorescence based assays. It can test both cells and media, both frozen and fresh samples. It does not require 2 weeks antibiotic free culture time. It takes about 5 to 6 hours to finish. The only not very convenient step is the 65C incubation in water bath.

Serum MycoProbe Mycoplasma Detection Kit CUL001B
MycoProbe Mycoplasma Detection Kit
By Anonymous on 12/06/2019
Application:
MycoProbe Mycoplasma Detection Kit
By Anonymous on 04/03/2018
Application:
MycoProbe Mycoplasma Detection Kit
By Anonymous on 07/07/2017
Application:
MycoProbe Mycoplasma Detection Kit
By Anonymous on 05/13/2016
Application:

Our lab has been using this kit for routine Mycoplasma testing of cultures for over 2 years now. The kit is highly sensitive and specific to Mycoplasma. The data is reproducible and more accurate than other tests, the size of the assay is adjustable (the kit comes as 12 8-well strips). You get reliable results in a few hours. Overall, great product and highly recommended.

Serum MycoProbe Mycoplasma Detection Kit CUL001B