Cultrex Human Fibronectin, PathClear

Name: Cultrex Human Fibronectin, PathClear
Brand: R&D Systems
SKU: 3420-001-01

R&D Systems | Catalog # 3420-001-01

Submit a review

Citations (1)

Product Documents

Discontinued Product

3420-001-01 has been discontinued. View all Fibronectin products.

Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents
Research Areas

Key Product Details

Features

Cultrex Human Fibronectin, Pathclear is a purified stromal extracellular matrix protein that has been qualified as a coating solution that promotes for cell attachment to tissue culture vessels.

Key Benefits

Qualifed as a coating substrate that promotes cell attachement
Can be used as a medium additive
Pathclear-tested to ensure product is free of human virus and pathogens
Source tissue is LDEV-free

Species

Human

Product Summary for Cultrex Human Fibronectin, PathClear

Why Use Cultrex Human Fibronectin?

Cultrex Human Fibronectin, Pathclear is purified from human plasma and is Pathclear certified to be free of common pathogens. Fibronectin is an extracellular matrix protein that is found abundantly in blood and connective tissues. These matrices are associated with the epithelial to mesenchymal transition of metastatic cells--including tumor cells with stem cell-like properties. Fibronectin performs essential functions in collagen fibrillogenesis, as either a general cell adhesion molecule or a modulator in binding between cell surfaces and the extracellular matrix. Fibronectin matrix assembly is essential for normal vertebrate development and apparently contributes to the generation of tumor metastases by supporting the establishment and persistence of pre-metastatic niches. Fibronectin is secreted as a disulfide-linked dimer of 230-270 kDa, comprised of three types of repeating modules that mediate interactions with extracellular matrix components (including fibronectin itself), and cells via integrins and other fibronectin receptors. Thus, fibronectin can be used for coating tissue culture surfaces or as a medium additive to promote cell adhesion and proliferation; it is 0.2 μm sterile filtered.

Product Specifications

Source

Human plasma

Protein Concentration

1 mg/mL

Endotoxin Level

≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Sterility Testing

Tested following USP 71> sterility guidelines.

Cell Culture Testing

Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.

Viral Testing

Tested negative by PCR test for mycoplasma and human pathogenic viruses, including: EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, and VZV.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Stability

Product is stable for a minimum of 3 months from date of shipment when stored at ≤ -20 °C. For optimal stability store at ≤ -70 °C. Avoid freeze-thaw cycles.

Background: Fibronectin

Fibronectin (FN) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. Fibronectin is a ligand for many molecules including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils. Conversion of soluble fibronectin to fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization.