Polylysine is a type of polyamino acid that can take several forms depending on the stereochemistry of the molecule. Polyamino acids can aid in the attachment of many cell types to tissue culture surfaces by serving as an exogenous source for extracellular matrix. Polylysine coating helps facilitate growth, cell spreading, motility, and differentiation of cells. Typically, polylysine comes in two conformations: Poly-L-Lysine and Poly-D-Lysine. Depending on the cell culture application and other reagents, Poly-L-Lysine or Poly-D-Lysine may be preferred for certain cell types.
Cultrex Poly-D-Lysine
R&D Systems | Catalog # 3439-200-01
Poly-amino solution that promotes cell adhesion
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Key Product Details
Features
Cultrex Poly-D-Lysine is a poly-amino acid solution that is provided ready-to-use at a concentration 0.01%.
Key Benefits
- Promotes the proliferation and differentiation of a variety of neuronal cell lines
- Facilitates cell adhesion on tissue culture-treated plastic or glass surfaces
- Contains polymers in the 70,000-150,000 Da range
- Provided in phosphate-buffered saline (PBS)
Product Summary for Cultrex Poly-D-Lysine
Why Use Cultrex Poly-D-Lysine?
Poly-D-Lysine, a highly positively charged synthetic amino acid chain, is commonly used as a coating agent to promote cell adhesion in culture on tissue culture-treated plastic or glass surfaces. Moreover, poly-D-Lysine is resistant to enzymatic degradation and promotes the proliferation and differentiation of a variety of neuronal cell lines. This solution contains polymers in the 70,000-150,000 Da range, and it is provided sterile filtered and ready to use at 0.01%.
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Product Specifications
Protein Concentration
0.1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Supports attachment of rat PC-12 pheochromocytoma cells.
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C.
Stability
Product is stable for at least 3 months from the date of receipt when stored at 2-8 °C. Keep sterile.
Background: Poly-D-Lysine
Alternate Names
PolyDLysine
Additional Poly-D-Lysine Products
Product Documents for Cultrex Poly-D-Lysine
Citations for Cultrex Poly-D-Lysine
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