Cultrex Rat Collagen I, Lower Viscosity

Purified stromal extracellular matrix protein that can promte cell adhesion for cell and tissue cultures
Catalog # Availability Size / Price Qty
3443-100-01
Product Details
Citations (3)
FAQs
Reviews

Cultrex Rat Collagen I, Lower Viscosity Summary

Cultrex Rat Collagen I, Low Viscosity is a purified stromal extracellular matrix protein that is formulated as an easy to work with hydrogel for coating tissue culture vessels. It may also be used to supplement customized coatings, hydrogels, or medium formulations for cell culture.

Key Benefits

• Qualified for general cell culture applications
• Lower in viscosity for easier handling
• May be used as a hydrogel or thin coating for tissue culture vessels
• Material provided at a standard concentration of ~3 mg/mL

Why Use Cultrex Rat Collagen I, Lower Viscosity?

Cultrex Rat Collagen I, Lower Viscosity is purified from rat tail tendons and is provided at a standard concentration that is lower in viscosity, making it easier to work with. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 3 mg/mL.

Specifications

Source
Rat tail tendons
Protein Concentration
3 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Stability
Product is stable for a minimum of 3 months if stored at 2-8 °C
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not freeze.
Species
Rat

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Cultrex Rat Collagen I, Lower Viscosity

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Boosting Drug Discovery for Parkinson's: Enhancement of the Delivery of a Monoamine Oxidase-B Inhibitor by Brain-Targeted PEGylated Polycaprolactone-Based Nanoparticles
    Authors: M Pinto, C Fernandes, E Martins, R Silva, S Benfeito, F Cagide, RF Mendes, FA Almeida Pa, J Garrido, F Remião, F Borges
    Pharmaceutics, 2019;11(7):.  2019
  2. Innovative in cellulo method as an alternative to in vivo neurovirulence test for the characterization and quality control of human live Yellow Fever virus vaccines: A pilot study
    Authors: A da Costa, C Prehaud, C Khou, N Pardigon, A Saulnier, N Nougarede, M Lafon
    Biologicals, 2018;0(0):.  2018
  3. Assessing the Impact of Targeting CEACAM1 in Head and Neck Squamous Cell Carcinoma
    Authors: K Tam, DW Schoppy, JH Shin, JK Tay, U Moreno-Nie, DC Mundy, JB Sunwoo
    Otolaryngol Head Neck Surg, 2018;0(0):1945998187566.  2018

FAQs

  1. What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    • Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  2. What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

    • Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.

  3. Is Cultrex Rat Collagen I atelocollagen or telocollagen?

    • Cultrex Rat Collagen I is telocollagen.  

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