Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
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Cultrex Rat Collagen I, Lower Viscosity
R&D Systems | Catalog # 3443-100-01
Purified stromal extracellular matrix protein that can promte cell adhesion for cell and tissue cultures
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Key Product Details
Features
Cultrex Rat Collagen I, Low Viscosity is a purified stromal extracellular matrix protein that is formulated as an easy to work with hydrogel for coating tissue culture vessels. It may also be used to supplement customized coatings, hydrogels, or medium formulations for cell culture.
Key Benefits
- Qualified for general cell culture applications
- Lower in viscosity for easier handling
- May be used as a hydrogel or thin coating for tissue culture vessels
- Material provided at a standard concentration of ~3 mg/mL
Species
Rat
Product Summary for Cultrex Rat Collagen I, Lower Viscosity
Why Use Cultrex Rat Collagen I, Lower Viscosity?
Cultrex Rat Collagen I, Lower Viscosity is purified from rat tail tendons and is provided at a standard concentration that is lower in viscosity, making it easier to work with. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 3 mg/mL.
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Product Specifications
Source
Rat tail tendons
Protein Concentration
3 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not freeze.
Stability
Product is stable for a minimum of 2 months if stored at 2-8 °C
Background: Collagen I
Alternate Names
Alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, Collagen 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain
Additional Collagen I Products
Product Documents for Cultrex Rat Collagen I, Lower Viscosity
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Citations for Cultrex Rat Collagen I, Lower Viscosity
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FAQs for Cultrex Rat Collagen I, Lower Viscosity
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Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
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Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
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Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
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Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
-
Q: Is Cultrex Rat Collagen I atelocollagen or telocollagen?
A: Cultrex Rat Collagen I is telocollagen.
-
Q: What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?
A: Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
-
Q: What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?
A: Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
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