Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

Basement membrane extract specifically qualified to support feeder-free stem cell culture for embryonic and pluripotent stem cells
Catalog # Availability Size / Price Qty
3434-001-02
3434-005-02
3434-010-02

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Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs).
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Citations (6)
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Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract Summary

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.
 

Key Benefits

• Qualified to support the culture of pluripotent stem cell
• Supports human iPSC and ESC expansion and differentiation
• Reduced growth factor formulation provides a more defined culture system
• Quality controlled for performance consistency

Why Use Cultrex Stem Cell Qualified RGF BME?

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective surface for feeder-free stem cell attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Promotes the human induced pluripotent stem cell attachment.

Effectively maintains human iPSCs in a pluripotent state in a feeder-free stem cell culture.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Data Example

Flow Cytometry Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). View Larger

Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34+, CD45+). Data courtesy of the Verneris Laboratory at the University of Colorado.

Stem Cell Colonies of Human iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. View Larger

Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core.

Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency
    Authors: KJ Choi, MD Quan, C Qi, JH Lee, PS Tsoi, M Zahabiyon, A Bajic, L Hu, BVV Prasad, SJ Liao, W Li, ACM Ferreon, JC Ferreon
    Nature Cell Biology, 2022;0(0):.  2022
  2. Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas
    Authors: MP Pappas, N Xie, JS Penaloza, SSK Chan
    Cells, 2022;11(9):.  2022
  3. Generation of two human iPSC lines, FINCBi002-A and FINCBi003-A, carrying heteroplasmic macrodeletion of mitochondrial DNA causing Pearson's syndrome
    Authors: C Peron, R Mauceri, A Iannielli, A Cavaliere, A Legati, A Rizzo, FL Sciacca, V Broccoli, V Tiranti
    Stem Cell Research, 2021;50(0):102151.  2021
  4. Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber�s Hereditary optic Neuropathy (LHON)
    Authors: C Peron, R Mauceri, T Cabassi, A Segnali, A Maresca, A Iannielli, A Rizzo, FL Sciacca, V Broccoli, V Carelli, V Tiranti
    Stem Cell Res, 2020;48(0):101939.  2020
  5. Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber�s Hereditary optic Neuropathy (LHON)
    Authors: C Peron, R Mauceri, T Cabassi, A Segnali, A Maresca, A Iannielli, A Rizzo, FL Sciacca, V Broccoli, V Carelli, V Tiranti
    Stem Cell Res, 2020;48(0):101939.  2020
  6. Doublecortin like kinase 1 regulates alpha-Synuclein levels and toxicity
    Authors: GE Vázquez-Vé, KA Gonzales, JP Revelli, CJ Adamski, F Alavi Nain, A Baji?, E Craigen, R Richman, SM Heman-Acka, MJA Wood, MWC Rousseaux, HY Zoghbi
    J. Neurosci., 2019;0(0):.  2019

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

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