Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

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Catalog # Availability Size / Price Qty
3434-001-02
3434-005-02
3434-010-02
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Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract Summary

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (RGF BME) has been shown to provide anprovides effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.

Key Benefits

• Qualified to support the culture of pluripotent stem cell
• Supports human iPSC and ESC expansion and differentiation
• Reduced growth factor formulation provides a more defined culture system
• Quality controlled for performance consistency

Why Use Cultrex Stem Cell Qualified RGF BME?

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective feeder-free surface for the attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
Promotes the attachment of human induced pluripotent stem cells (iPSCs).

Effectively manintains human iPSCs in a pluripotent state in a feeder-free culture.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for two years from date of manufacture. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

FAQs

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