CYBB/NOX2 Antibody (SN07-16)

Novus Biologicals | Catalog # NBP2-67680

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN07-16 expressed in HEK293
View all CYBB/NOX2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CYBB/NOX2 aa 131-180 / 570. (SwissProt: P04839 Human; SwissProt: Q61093 Mouse; Entrez Gene:66021 Rat)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYBB/NOX2 Antibody (SN07-16)

CYBB/NOX2 Antibody (SN07-16) Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680]

Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680]

Western blot analysis on different lysates with NBP2-67680 at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67680 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for CYBB/NOX2 Antibody (SN07-16)

Application
Recommended Usage

Western Blot

1:2,000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYBB/NOX2

Long Name

Cytochrome b-245 heavy chain

Alternate Names

CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2

Gene Symbol

CYBB

Additional CYBB/NOX2 Products

Product Documents for CYBB/NOX2 Antibody (SN07-16)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CYBB/NOX2 Antibody (SN07-16)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYBB/NOX2 Antibody (SN07-16)

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