During the G1 phase of the cell cycle, a cell must choose between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.
Rat Cyclin D2 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-75105
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
18.75 pg/mL
Assay Range
31.25 - 2000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat CCND2 in samples. No significant cross-reactivity or interference between Rat CCND2 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 4.71%
Inter-Assay Precision (Precision between assays) CV% 4.84%
Recovery for Rat Cyclin D2 ELISA Kit (Colorimetric)
Recovery
84-104%
Linearity
Scientific Data Images for Rat Cyclin D2 ELISA Kit (Colorimetric)
ELISA: Rat Cyclin D2 ELISA Kit (Colorimetric) [NBP2-75105] -
ELISA: Rat Cyclin D2 ELISA Kit (Colorimetric) [NBP2-75105] - Standard Curve ReferenceKit Contents for Rat Cyclin D2 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Cyclin D2
Alternate Names
CCND2, Vin1
Gene Symbol
CCND2
Additional Cyclin D2 Products
Product Documents for Rat Cyclin D2 ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rat Cyclin D2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
