Rat Cyclin E2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-75111

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
View all Cyclin E2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat CCNE2 in samples. No significant cross-reactivity or interference between Rat CCNE2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.95%

Inter-Assay Precision (Precision between assays) CV% 5.25%

Recovery for Rat Cyclin E2 ELISA Kit (Colorimetric)

Recovery

86-106%

Linearity

Rat Cyclin E2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Cyclin E2 ELISA Kit (Colorimetric)

Rat Cyclin E2 ELISA Kit (Colorimetric)

ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] -

ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] - Standard Curve Reference

Kit Contents for Rat Cyclin E2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cyclin E2

Cyclin E2 (CCNE2) is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (aa 141-231 and aa 241-361). An alternate splice form lacks 45 amino acids within the first cyclin box fold.

Alternate Names

CCNE2, CYCE2

Gene Symbol

CCNE2

Additional Cyclin E2 Products

Product Documents for Rat Cyclin E2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Cyclin E2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cell Cycle

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