Recombinant Human Cyclophilin-E His Protein

Name: Recombinant Human Cyclophilin-E His Protein
Brand: Novus Biologicals
SKU: NBC1-18531
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBC1-18531

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE

View all Cyclophilin-E Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-301 of Human Cyclophilin-E

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 210 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cyclophilin-E His Protein

SDS-PAGE: Recombinant Human Cyclophilin-E His Protein [NBC1-18531]

SDS-Page: Cyclophilin-E Protein [NBC1-18531] - PPIE, 37.5 kDa (337 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18531

Formulation	20 mM Tris-HCl buffer (pH 8.0)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin-E

Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE. Recombinant human PPIE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

Cyclophilin E, Cyclophilin-33, CYP33, CYP-33, EC 5.2.1.8, MGC111222, MGC3736, peptidyl-prolyl cis-trans isomerase E, peptidylprolyl isomerase E (cyclophilin E), PPIase E, Rotamase E

Gene Symbol

PPIE

Additional Cyclophilin-E Products

Product Documents for Recombinant Human Cyclophilin-E His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Cyclophilin-E His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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