Recombinant Human Cyclophilin-F His Protein

Novus Biologicals | Catalog # NBC1-18533

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Western Blot, Immunoprecipitation, Bioactivity, SDS-PAGE
View all Cyclophilin-F Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-207 of Human Cyclophilin-F

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Application Notes

Use in immunoprecipitation, and western blot reported in scientific literature (PMID 26387735)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cyclophilin-F His Protein

SDS-PAGE: Recombinant Human Cyclophilin-F His Protein [NBC1-18533]

SDS-PAGE: Recombinant Human Cyclophilin-F His Protein [NBC1-18533]

SDS-Page: Recombinant Human Cyclophilin-F Protein [NBC1-18533] - Cyclophilin F(PPIF), 21 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18533
Formulation 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin-F

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Cyclophilin F, CYP3FLJ90798, Cyp-D, hCyP3, MGC117207, mitochondrial, peptidyl-prolyl cis-trans isomerase, mitochondrial, peptidylprolyl isomerase F, peptidylprolyl isomerase F (cyclophilin F)

Gene Symbol

PPIF

Additional Cyclophilin-F Products

Product Documents for Recombinant Human Cyclophilin-F His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cyclophilin-F His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Cyclophilin-F His Protein

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