Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80685
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3A7.1B8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Partial recombinant protein from human Cyr61/CCN1 protein (between residues 100-300). [Swiss-Prot O00622]
Reactivity Notes
Immunogen's sequence similarity with other species: Rat (81%), Mouse (79%), Camel (88%), Naked Mole Rat (87%), Porcine (80%), Bovine / Sheep / Horse (82%), Chicken (63%)
Localization
Secreted (Cytoplasmic/Extracellular)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Novus Biologicals Mouse Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free (NBP2-36490) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free
Western Blot: Cyr61/CCN1 Antibody (3A7.1B8)Azide and BSA Free [NBP2-80685]
Western Blot: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685] - WB analysis of (A) 12kDa Partial Recombinant Cyr61/CCN1 protein and (B) Human Liver lysate using Cyr61/CCN1 antibody (clone 3A7.1B8) at 4ug/ml concentration. Image from the standard format of this antibody.Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685]
Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The hepatocytes generated a specific cytoplasmic staining with no signal in the cellular nuclei or the sinusImmunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685]
Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human hepatocellular carcinoma using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The cancer cells generated a specific cytoplasmic staining with some staining in the inteImmunohistochemistry: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685]
Immunohistochemistry: Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free [NBP2-80685] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human Renal Cell Carcinoma using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The cancer cells generated a specific cytoplasmic staining with some staining in the inter-ceApplications for Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
4 ug/ml
Application Notes
Protein CYR61 is a secreted protein and its expected immunolocalization would be cytoplasmic as well as the extracellular spaces.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cyr61/CCN1
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Alternate Names
CCN1, GIG1, IGFBP-10
Gene Symbol
CCN1
Additional Cyr61/CCN1 Products
Product Documents for Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free
Product Specific Notices for Cyr61/CCN1 Antibody (3A7.1B8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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