Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-18022

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31-20 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.95

Inter-Assay Precision (Precision between assays) %CV 6.10

Recovery for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

Recovery

83-104%

Linearity

Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

ELISA: Human Cytochrome P450 1A1 ELISA Kit (Colorimetric) [NBP3-18022] -

ELISA: Human Cytochrome P450 1A1 ELISA Kit (Colorimetric) [NBP3-18022] - Standard Curve Reference

Kit Contents for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cytochrome P450 1A1

The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Long Name

Cytochrome P450 1A1

Alternate Names

CYP1A1, CYPIA1, Cytochrome P450-C, Cytochrome P450-P1

Gene Symbol

CYP1A1

Additional Cytochrome P450 1A1 Products

Product Documents for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Cytochrome P450 1A1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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