Cytochrome P450 2E1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-85366
![Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Western-Blot-NBP1-85366-img0004.jpg "Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLIL
Reactivity Notes
Reactivity reported in scientific literature (PMID: 24309898)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytochrome P450 2E1 Antibody - BSA Free
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366]
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0012.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver shows strong cytoplasmic positivity in tumor cells.![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0008.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85366 (A) shows similar protein distribution across tissues to independent antibody NBP1-85367 (B).![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0009.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0010.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human tonsil shows no positivity in germinal center cells as expected.![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0011.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium shows no positivity in glandular cells as expected.![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]](https://resources.rndsystems.com/images/products/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85366-img0013.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]")
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver cancer, hepatocellular carcinoma shows moderate cytoplasmic positivity in tumor cells.Applications for Cytochrome P450 2E1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytochrome P450 2E1
Alternate Names
4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase
Gene Symbol
CYP2E1
Additional Cytochrome P450 2E1 Products
Product Documents for Cytochrome P450 2E1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytochrome P450 2E1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Cytochrome P450 2E1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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