Cytokeratin 6a Antibody (SN71-07)

Novus Biologicals | Catalog # NBP2-67705

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN71-07 expressed in HEK293
View all Cytokeratin 6a Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Cytokeratin 6a aa 1-118 / 564. (SwissProt: P02538 Human; SwissProt: P04259 Human; SwissProt: P48668 Human; SwissProt: Q4FZU2 Rat)

Localization

Keratin filament, cytosol, extracellular exosome, nucleus, membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 6a Antibody (SN71-07)

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705] - Analysis of Cytokeratin 6a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Human skin tissue lysate
Immunocytochemistry/ Immunofluorescence: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunocytochemistry/Immunofluorescence: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705] - Staining Cytokeratin 6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 6a (washed with PBS). And then probed with the primary antibodies Cytokeratin 6 (red) at 1/50 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705] - Analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 6 antibody. Counter stained with hematoxylin.
Cytokeratin 6a Antibody (SN71-07) Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western Blot: Cytokeratin 6a Antibody (SN71-07) [NBP2-67705]

Western blot analysis on different lysates with NBP2-67705 at 1/1,000 dilution. Lane 1: HaCaT cell lysate Lane 2: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67705 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Cytokeratin 6a Antibody (SN71-07)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 6a

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Alternate Names

Allergen Hom s 5, CK6A, CK-6A, CK6C, CK6D, CK-6D, cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, Cytokeratin-6A, Cytokeratin-6D, K6A, K6C, K6D, keratin 6A, keratin 6C, keratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, Keratin-6A, KRT6C, KRT6D, Type-II keratin Kb6

Gene Symbol

KRT6A

Additional Cytokeratin 6a Products

Product Documents for Cytokeratin 6a Antibody (SN71-07)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 6a Antibody (SN71-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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