D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

Novus Biologicals | Catalog # NB100-56556

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Equine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 10D774

Format

BSA Free
View all D4-GDI/RhoGDI2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.
Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI.
Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556]

Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.

Applications for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 25623267)

Western Blot

1-2 ug/ml
Application Notes
A 28 kDa band is observed.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.02% Gelatin

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: D4-GDI/RhoGDI2

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Alternate Names

ARHGDIB Rho GDP dissociation inhibitor (GDI) beta, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2

Gene Symbol

ARHGDIB

Additional D4-GDI/RhoGDI2 Products

Product Documents for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

Certificate of Analysis

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Product Specific Notices for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for D4-GDI/RhoGDI2 Antibody (10D774) - BSA Free

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Protocols

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