DAPK3/ZIPK Antibody (PSH02-18)

Novus Biologicals | Catalog # NBP3-33113

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH02-18 expressed in HEK293
View all DAPK3/ZIPK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human DAPK3/ZIPK aa 250-454 (O43293). (Uniprot: O43293)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAPK3/ZIPK Antibody (PSH02-18)

DAPK3/ZIPK Antibody (PSH02-18) Western Blot: DAPK3/ZIPK Antibody (PSH02-18) [NBP3-33113]

Western Blot: DAPK3/ZIPK Antibody (PSH02-18) [NBP3-33113]

Western blot analysis on different lysates with NBP3-33113 at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: HepG2 cell lysate Lane 6: Hela cell lysate Lane 7: Jurkat cell lysate Lane 8: Caco-2 cell lysate Lane 9: K-562 cell lysate Lane 10: MCF7 cell lysate Lane 11: U-2 OS cell lysate Lane 12: Human kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33113 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for DAPK3/ZIPK Antibody (PSH02-18)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAPK3/ZIPK

DAPK3, a DAPK type protein kinase, is functions in apoptotic signaling and also is believed to function in coordination of specific transcription and splicing events. DAPK3 contains a leucine zipper motif at its C terminus in addition to the N terminal kinase domain. DAPK3 binds to ATF4, a member of the activating transcription factor/cyclic AMP-responsive element-binding protein (ATF/CREB) family.

Long Name

Death-associated Protein Kinase 3

Alternate Names

Dlk, ZIPK

Gene Symbol

DAPK3

Additional DAPK3/ZIPK Products

Product Documents for DAPK3/ZIPK Antibody (PSH02-18)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DAPK3/ZIPK Antibody (PSH02-18)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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