Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein

Name: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP3-06720
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-06720

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

Strep (N-Term)

Applications

ELISA, Flow Cytometry

View all Daucus carota 1.0101 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal Strep-tag corresponding to 167 amino acids of Daucus carota 1.0101

Source: E.coli

Store product aliquots at -20C to -70C. Use stored aliquots within 6 months.

Purity

>95%, by HPLC

Predicted Molecular Mass

17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein

Flow Cytometry: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720]

Flow Cytometry: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720] - Flow cytometry dot-plot staining pattern of Daucus carota 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.

ELISA: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720]

ELISA: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720] - ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen Daucus carota 1.0101 and allergen-specific human plasma IgG4 antibodies of Daucus carota positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Daucus carota 1.0101.

SDS-PAGE: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720]

SDS-Page: Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein [NBP3-06720] - Recombinant allergen Daucus carota 1.0101 purity verification. 5 ug of Daucus carota 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Formulation, Preparation, and Storage

NBP3-06720

Preparation Method	The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Formulation	100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution	Reconstitute with sterile deionized water with or without azide.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Daucus carota 1.0101

Alternate Names

rDau c 1

Additional Daucus carota 1.0101 Products

Product Documents for Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Plant Daucus carota 1.0101 Strep (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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