DCK Antibody (10412) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43639

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10412

Format

Azide and BSA Free
View all DCK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DCK Antibody (10412) - Azide and BSA Free

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (10412) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: DCK Antibody (10412) [NBP2-43639]

Immunocytochemistry/ Immunofluorescence: DCK Antibody (10412) [NBP2-43639]

Immunocytochemistry/Immunofluorescence: DCK Antibody (10412) [NBP2-43639] - DCK antibody (10412) detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639]

Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639]

Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human lung cancer. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.
Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639]

Western Blot: DCK Antibody (10412) [NBP2-43639] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639]

Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639]

Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human colon carcinoma. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: DCK Antibody (10412) [NBP2-43639]

Immunoprecipitation: DCK Antibody (10412) [NBP2-43639]

Immunoprecipitation: DCK Antibody (10412) [NBP2-43639] - Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 ug of DCK antibody (10412) or DCK antibody (NBP2-16108). Western blot analysis was performed using DCK antibody (10412) diluted at 1:500.

Applications for DCK Antibody (10412) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-43639 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DCK

DCK (Deoxycytidine kinase) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Long Name

Deoxycytidine kinase

Alternate Names

EC 2.7.1.113, EC 2.7.1.74, EC 2.7.1.76

Gene Symbol

DCK

Additional DCK Products

Product Documents for DCK Antibody (10412) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DCK Antibody (10412) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DCK Antibody (10412) - Azide and BSA Free (1)

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  • DCK Antibody (10412)
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: 3 human cancer cell lines
    Species: Human
    Verified Customer | Posted 09/14/2017
    3 tumor cell lines (10ug lysate) are loaded. 1/2000 dil in 2% BSA/TBST, incubated for o/n at 4C
    DCK Antibody (10412) - Azide and BSA Free NBP2-43639

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