DCTN3 Antibody

Novus Biologicals | Catalog # NBP1-52105

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DCTN3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QVALGNISSKRE corresponding to internal region according to NP_009165.1; NP_077324.1.

Epitope

C-QVALGNISSKRE

Specificity

This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DCTN3 Antibody

Western Blot: DCTN3 Antibody [NBP1-52105]

Western Blot: DCTN3 Antibody [NBP1-52105]

Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.03ug/ml) of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105]

Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105]

Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Flow Cytometry: DCTN3 Antibody [NBP1-52105]

Flow Cytometry: DCTN3 Antibody [NBP1-52105]

Flow Cytometry: DCTN3 Antibody [NBP1-52105] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: DCTN3 Antibody [NBP1-52105]

Western Blot: DCTN3 Antibody [NBP1-52105]

Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.2ug/ml) of Mouse (A) and Rat (B) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105]

Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105]

Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Staining (8ug/ml) of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Applications for DCTN3 Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunohistochemistry-Paraffin

6 - 8 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx 20 kDa band observed in Human Liver lysates, and approx. 18 kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19. 5kDa according to NP_077324.1 and 21.1 kDa according to NP_001102129.1.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DCTN3

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.

Alternate Names

DCTN22DCTN-22, dynactin 3, dynactin 3 (p22), Dynactin complex subunit 22 kDa subunit, dynactin light chain, dynactin subunit 3, MGC111190, p22

Entrez Gene IDs

11258 (Human); 53598 (Mouse); 362504 (Rat)

Gene Symbol

DCTN3

UniProt

NP_009165.1 (Human)

Additional DCTN3 Products

Product Documents for DCTN3 Antibody

Certificate of Analysis

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Product Specific Notices for DCTN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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