Deazaflavin, Haloalkane

Tocris Bioscience | Catalog # 8987

Photosensitizer for proximity labeling experiments, NHS reactive group
Tocris Bioscience

Key Product Details

Description

Photosensitizer for proximity labeling experiments, NHS reactive group
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Product Description

Deazaflavin, Haloalkane is a deazaflavin-based organic photocatalyst covalently linked to a chloroalkane ligand via a short PEG linker. The conjugate enables site-specific tethering of the photocatalyst to proteins genetically fused to HaloTag® in live-cell systems.

Upon blue light illumination (~450 nm), the deazaflavin moiety transfers energy to nearby photoactivatable probes (e.g., diazirines, aryl azides), initiating localized covalent labeling of biomolecules in the immediate vicinity of the HaloTag® fusion protein. The process is dependent on light exposure, the presence of the reactive probe, and spatial proximity to the tethered photocatalyst.

Deazaflavin, Haloalkane is designed for use in photoproximity labeling experiments that aim to study protein interactions or spatial proteomics in live mammalian cells under mild illumination conditions without the use of metal-based photocatalysts.

HaloTag® is a registered trademark of Promega Corporation.

Product Specifications for Deazaflavin, Haloalkane

Molecular Weight

490.99

Formula

C24H31ClN4O5

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-(2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)-2-(10-methyl-2,4-dioxo-4,10-dihydropyrimido[4,5-b]quinolin-3(2H)-yl)acetamide

InChI Key

CFRUFYBIYIESMB-UHFFFAOYSA-N

SMILES

O=C1N(C(C2=CC3=CC=CC=C3N(C2=N1)C)=O)CC(NCCOCCOCCCCCCCl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.91 10

Preparing Stock Solutions for Deazaflavin, Haloalkane

The following data is based on the product molecular weight 490.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.37 mL 101.84 mL 203.67 mL
0.5 mM 4.07 mL 20.37 mL 40.73 mL
1 mM 2.04 mL 10.18 mL 20.37 mL
5 mM 0.41 mL 2.04 mL 4.07 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for Deazaflavin, Haloalkane

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Deazaflavin, Haloalkane

For research use only

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