Deazaflavin, Haloalkane

Catalog # Availability Size / Price Qty
8987/50U

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Deazaflavin, Haloalkane | Buffers, Chelators and Reagents
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Description: Photosensitizer for proximity labeling experiments, NHS reactive group

Chemical Name: N-(2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)-2-(10-methyl-2,4-dioxo-4,10-dihydropyrimido[4,5-b]quinolin-3(2H)-yl)acetamide

Purity: ≥95%

Product Details
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Biological Activity

Deazaflavin, Haloalkane is a deazaflavin-based organic photocatalyst covalently linked to a chloroalkane ligand via a short PEG linker. The conjugate enables site-specific tethering of the photocatalyst to proteins genetically fused to HaloTag® in live-cell systems.

Upon blue light illumination (~450 nm), the deazaflavin moiety transfers energy to nearby photoactivatable probes (e.g., diazirines, aryl azides), initiating localized covalent labeling of biomolecules in the immediate vicinity of the HaloTag® fusion protein. The process is dependent on light exposure, the presence of the reactive probe, and spatial proximity to the tethered photocatalyst.

Deazaflavin, Haloalkane is designed for use in photoproximity labeling experiments that aim to study protein interactions or spatial proteomics in live mammalian cells under mild illumination conditions without the use of metal-based photocatalysts.

HaloTag® is a trademark of Promega Corporation.

Technical Data

M.Wt:
490.99
Formula:
C24H31ClN4O5
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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