Recombinant Human Deoxycytidylate deaminase His Protein

Name: Recombinant Human Deoxycytidylate deaminase His Protein
Brand: Novus Biologicals
SKU: NBP1-45280
Price: 349 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-45280

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Deoxycytidylate deaminase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-178 of Human Deoxycytidylate deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Deoxycytidylate deaminase His Protein

Western Blot: Recombinant Human Deoxycytidylate deaminase His Protein [NBP1-45280]

Western Blot: Deoxycytidylate deaminase Protein [NBP1-45280] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-45280

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 1 mM DTT, 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Deoxycytidylate deaminase

DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. Using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to dUMP, thereby producing the nucleotide substrate (dUMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

dCMP deaminaseEC 3.5.4.12, deoxycytidylate deaminase, MGC111062

Gene Symbol

DCTD

Additional Deoxycytidylate deaminase Products

Product Documents for Recombinant Human Deoxycytidylate deaminase His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Deoxycytidylate deaminase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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