Desmocollin-1 Antibody (JE66-57)

Novus Biologicals | Catalog # NBP3-32264

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE66-57 expressed in HEK293
View all Desmocollin-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Desmocollin-1 aa 795-894 / 894. (Uniprot: Q08554)

Localization

Cell membrane, Cell junction, desmosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmocollin-1 Antibody (JE66-57)

Desmocollin-1 Antibody (JE66-57)

Western Blot: Desmocollin-1 Antibody (JE66-57) [NBP3-32264]

Western Blot: Desmocollin-1 Antibody (JE66-57) [NBP3-32264] - Western blot analysis of Desmocollin-1 on different lysates with Rabbit anti-Desmocollin-1 antibody (NBP3-32264) at 1/1,000 dilution.

Lane 1: C2C12 cell lysate
Lane 2: L6 cell lysate
Lane 3: RH-35 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 100 kDa
Observed band size: 100/60kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32264) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Desmocollin-1 Antibody (JE66-57)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmocollin-1

The protein encoded by the Desmosomal Protein gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Alternative splicing results in two transcript variants encoding distinct isoforms. (provided by RefSeq)

Alternate Names

CDHF1, Desmocollin1, DG2/DG3, DSC1

Gene Symbol

DSC1

Additional Desmocollin-1 Products

Product Documents for Desmocollin-1 Antibody (JE66-57)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Desmocollin-1 Antibody (JE66-57)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cadherin Superfamily

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