DFF40/CAD Antibody (36A349) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80696

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 36A349

Format

Azide and BSA Free
View all DFF40/CAD Primary Antibodies »

Product Specifications

Immunogen

Anti-DFF40 monoclonal antibody was raised against a peptide corresponding to amino acids 205 to 222 of human DFF40.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24051182).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for DFF40/CAD Antibody (36A349) - Azide and BSA Free

Western Blot: DFF40/CAD Antibody (36A349)Azide and BSA Free [NBP2-80696]

Western Blot: DFF40/CAD Antibody (36A349)Azide and BSA Free [NBP2-80696]

Western Blot: DFF40/CAD Antibody (36A349) - Azide and BSA Free [NBP2-80696] - analysis of 30 ug of HeLa cell lysate with this antibody. Image from the standard format of this antibody.

Applications for DFF40/CAD Antibody (36A349) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DFF40/CAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribo-nuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 (Cat. # IMG-114 & 115) and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspases, DFF40/CAD is released and activated and eventually causes degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Long Name

DNA Fragmentation Factor, Subunit beta

Alternate Names

CAD, CPAN, DFF2, DFFB

Gene Symbol

DFFB

Additional DFF40/CAD Products

Product Documents for DFF40/CAD Antibody (36A349) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DFF40/CAD Antibody (36A349) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

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FAQs for DFF40/CAD Antibody (36A349) - Azide and BSA Free

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  • Q: Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?

    A: CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

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