Q: Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?

A: CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Human DFF40/CAD ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-74965

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human CAD in samples. No significant cross-reactivity or interference between Human CAD and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.46%

Inter-Assay Precision (Precision between assays) CV% 5.43%

Recovery for Human DFF40/CAD ELISA Kit (Colorimetric)

Recovery

87-102%

Linearity

Scientific Data Images for Human DFF40/CAD ELISA Kit (Colorimetric)

ELISA: Human DFF40/CAD ELISA Kit (Colorimetric) [NBP2-74965] -

ELISA: Human DFF40/CAD ELISA Kit (Colorimetric) [NBP2-74965] - Standard Curve Reference

Kit Contents for Human DFF40/CAD ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: DFF40/CAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Long Name

DNA Fragmentation Factor, Subunit beta

Alternate Names

CAD, CPAN, DFF2, DFFB

Gene Symbol

DFFB

Additional DFF40/CAD Products

Product Documents for Human DFF40/CAD ELISA Kit (Colorimetric)

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Download Manual - Human DFF40/CAD ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human DFF40/CAD ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

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