DGK-beta Antibody (OTI1F9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70552

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F9

Format

Azide and BSA Free
View all DGK-beta Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DGKB (NP_663733) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGK-beta Antibody (OTI1F9) - Azide and BSA Free

Western Blot: DGK-beta Antibody (OTI1F9)Azide and BSA Free [NBP2-70552]

Western Blot: DGK-beta Antibody (OTI1F9)Azide and BSA Free [NBP2-70552]

Western Blot: DGK-beta Antibody (OTI1F9) - Azide and BSA Free [NBP2-70552] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB.
Western Blot: DGK-beta Antibody (OTI1F9)Azide and BSA Free [NBP2-70552]

Western Blot: DGK-beta Antibody (OTI1F9)Azide and BSA Free [NBP2-70552]

Western Blot: DGK-beta Antibody (OTI1F9) - Azide and BSA Free [NBP2-70552] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.

Applications for DGK-beta Antibody (OTI1F9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGK-beta

Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. (provided by RefSeq)

Long Name

Diacylglycerol Kinase, beta 90kDa

Alternate Names

DAGK2, DGKB, DGKbeta

Gene Symbol

DGKB

Additional DGK-beta Products

Product Documents for DGK-beta Antibody (OTI1F9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DGK-beta Antibody (OTI1F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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FAQs

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