DIAPH-2 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-280

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DIAPH-2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Diaphanous Homolog 2 using the numbering given in entry NP_006720.1 (GeneID 1730).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIAPH-2 Antibody - BSA Free

Western Blot: DIAPH-2 Antibody [NB600-280]

Western Blot: DIAPH-2 Antibody [NB600-280]

Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.
Western Blot: DIAPH-2 Antibody [NB600-280]

Western Blot: DIAPH-2 Antibody [NB600-280]

Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.

Applications for DIAPH-2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
This antibody can be used for Western blot. *Suggested Working Dilutions: Western blot: 1:1,000-1:10,000 Immunoprecipitation: not tested Immunohistochemistry: not tested *Optimal working dilutions should be determined experimentally by the investigator.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DIAPH-2

This gene may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Entrez Gene IDs

1730 (Human)

Gene Symbol

DIAPH2

UniProt

O60879

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DIAPH-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DIAPH-2 Antibody - BSA Free

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